Rufyikiri Gervais, Thiry Yves, Wang Lian, Delvaux Bruno, Declerck Stéphane
Belgian Nuclear Research Centre (SCK°CEN), Radiation Protection Research Department, Radioecology Section, Boeretang 200, 2400 Mol, Belgium.
Belgian Nuclear Research Centre (SCK°CEN), Waste and Disposal Department, Boeretang 200, 2400 Mol, Belgium.
New Phytol. 2002 Nov;156(2):275-281. doi: 10.1046/j.1469-8137.2002.00520.x.
• Uranium (U) uptake and translocation by the arbuscular mycorrhizal (AM) fungus Glomus intraradices were studied under root-organ culture conditions with Agrobacterium rhizogenes -transformed carrot ( Daucus carota ) roots as host. • Two-compartment Petri plates were used to spatially separate a root compartment (RC) and a hyphal compartment (HC); root growth was restricted to the RC while extraradical hyphae grew in both RC and HC. The HC was labelled with 0.1 µM U at different pH conditions. At the end of the experiment, U was measured in the RC and in the HC. • The U absorption by the AM fungus was observed. It included; U uptake by the mycelium developing in the HC, and U translocation from the HC to the RC. The magnitude of this uptake and translocation was highly influenced by the pH of the growth medium, while translocation was highly correlated with the number of hyphae crossing the partition separating the two compartments. • These results are the first to show that an AM fungus can take up and translocate U towards roots.
• 在根器官培养条件下，以发根农杆菌转化的胡萝卜（胡萝卜属）根为宿主，研究了丛枝菌根（AM）真菌根内球囊霉对铀（U）的吸收和转运。
• 使用两室培养皿在空间上分隔根室（RC）和菌丝室（HC）；根生长限制在RC中，而根外菌丝在RC和HC中都生长。在不同pH条件下，用0.1 μM铀标记HC。实验结束时，测量RC和HC中的铀含量。
• 观察到AM真菌对铀的吸收情况。这包括：在HC中生长的菌丝体对铀的吸收，以及铀从HC向RC的转运。这种吸收和转运的程度受到生长培养基pH值的高度影响，而转运与穿过分隔两个室的隔板的菌丝数量高度相关。
• 这些结果首次表明，AM真菌能够吸收铀并将其向根转运。
