Sharples J M, Meharg A A, Chambers S M, Cairney J W G
Mycorrhiza Research Group, School of Science, Food and Horticulture, University of Western Sydney, Parramatta Campus, Locked Bag 1797, PENRITH SOUTH DC, NSW 1797, Australia.
Institute of Terrestrial Ecology, Monks Wood, Abbots Ripton, Huntingdon, Cambridgeshire, PE17 2LS, UK.
New Phytol. 2001 Jul;151(1):265-270. doi: 10.1046/j.1469-8137.2001.00146.x.
• Differential resistance to arsenate (AsO ) is demonstrated here among populations of the ericoid mycorrhizal fungus Hymenoscyphus ericae isolated from Calluna vulgaris in natural heathland soils and soils contaminated with AsO . • Isolates (c. 25) of the fungus from each of two As and Cu mine sites, and a natural heathland site, were screened for AsO and Cu resistance by growing isolates in media containing a range of AsO and Cu concentrations. • H. ericae populations from the mine sites demonstrated resistance to AsO compared with the heathland population; the mine-site populations producing significant growth at the highest AsO concentration (4.67 mol m ), whereas growth of the heathland population was almost completely inhibited. EC values for mine-site isolates were estimated to be 5-41-times higher than the heathland population. All isolates produced identical responses to increasing Cu concentrations, with no differences observed between mine-site and heathland isolates. • Populations of H. ericae on the contaminated mine sites have developed adaptive resistance to AsO . By contrast, Cu resistance appears to be constitutive.
• 本文展示了从天然石南荒地土壤和被砷酸盐污染的土壤中分离自帚石楠的类欧石楠菌根真菌格氏 Hymenoscyphus ericae 种群对砷酸盐（AsO₄³⁻）的抗性差异。
• 通过在含有一系列砷酸盐和铜浓度的培养基中培养分离物，对来自两个砷和铜矿山场地以及一个天然石南荒地场地的真菌分离物（约25个）进行了砷酸盐和铜抗性筛选。
• 与石南荒地种群相比，来自矿山场地的格氏 Hymenoscyphus ericae 种群表现出对砷酸盐的抗性；矿山场地种群在最高砷酸盐浓度（4.67 μmol m⁻³）下能显著生长，而石南荒地种群的生长几乎完全受到抑制。矿山场地分离物的EC值估计比石南荒地种群高5至41倍。所有分离物对铜浓度增加的反应相同，矿山场地和石南荒地分离物之间未观察到差异。
• 受污染矿山场地的格氏 Hymenoscyphus ericae 种群已对砷酸盐产生适应性抗性。相比之下，铜抗性似乎是组成型的。
