Sharples J M, Chambers S M, Meharg A A, Cairney J W G
1 Mycorrhiza Research Group, School of Science, University of Western Sydney, PO Box 10, Kingswood NSW 2747, Australia.
New Phytol. 2000 Oct;148(1):153-162. doi: 10.1046/j.1469-8137.2000.00734.x.
A total of 107 putative ericoid mycorrhizal endophytes were isolated from hair roots of Calluna vulgaris from two abandoned arsenic/copper mine sites and a natural heathland site in southwest England. The endophytes were initially grouped as 14 RFLP types, based on the results of ITS-RFLP analysis using the restriction endonucleases Hinf I, Rsa I and Hae III. ITS sequences were obtained for representative isolates from each RFLP type and compared phylogenetically with sequences for known ericoid mycorrhizal endophytes and selected ascomycetes. The majority of endophyte isolates (62-92%) from each site were identified as Hymenoscyphus ericae, but a number of other less common mycorrhizal RFLP types were also identified, all of which appear to have strong affinities with the order Leotiales. None of the less common RFLP types was isolated from C. vulgaris at more than one field site. Neighbour-joining analysis indicated similarities between the endophytes from C. vulgaris and mycorrhizal endophytes isolated from other Ericaceae and Epacridaceae hosts in North America and Australia.
从英格兰西南部两个废弃的砷/铜矿场以及一个天然石南荒原的欧石楠发根中总共分离出107种假定的石楠类菌根内生真菌。基于使用限制性内切酶Hinf I、Rsa I和Hae III进行的ITS-RFLP分析结果，这些内生真菌最初被分为14种RFLP类型。从每种RFLP类型中选取代表性分离株获取ITS序列，并与已知石楠类菌根内生真菌和选定子囊菌的序列进行系统发育比较。每个位点的大多数内生真菌分离株（62%-92%）被鉴定为欧石楠小煤炱，但也鉴定出一些其他不太常见的菌根RFLP类型，所有这些类型似乎与柔膜菌目有很强的亲缘关系。没有一种不太常见的RFLP类型在多个野外地点的欧石楠中被分离出来。邻接法分析表明，欧石楠内生真菌与从北美和澳大利亚其他杜鹃花科和澳石南科宿主中分离出的菌根内生真菌之间存在相似性。
