Graham J, Marshall B, Squire G R
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK.
New Phytol. 2003 Mar;157(3):667-675. doi: 10.1046/j.1469-8137.2003.00693.x.
• The distribution is examined of molecular (random amplified polymorphic DNA) markers within and between 12 wild raspberry (Rubus ideaus) populations ranging over 600 m altitude and 40 km distance from a lowland area of extensive raspberry cultivation to remote upland sites. • Most individuals are distinct, suggesting that seedling recruitment is the main means of propagation, but mean genetic similarities within sites (> 80%) are much greater than between sites (> 50%), suggesting a hindrance to gene movement between sites. • Sites of genetically distinct populations can be grouped in lowland, valley and upland clusters; genetic similarity between sites decreased at slightly > 4% per 100 m difference in altitude from c. 80% between adjacent sites to 50% at 600 m altitude difference. • Together with physiological data collected previously, the molecular evidence confirms that wild populations are more diverse than cultivars and suggests little gene flow from cultivated to wild, a result perhaps of reproductive asynchrony and little opportunity for seedling recruitment in established populations. The cause of the genetic differentiation between sites is not known and requires further study.
• 研究了12个野生覆盆子（悬钩子属）种群内部和之间分子（随机扩增多态性DNA）标记的分布情况，这些种群分布在海拔跨度600米、距离从大面积覆盆子种植的低地地区到偏远高地地点达40公里的范围内。
• 大多数个体是不同的，这表明实生苗补充是主要的繁殖方式，但各地点内的平均遗传相似性（>80%）远大于各地点间的平均遗传相似性（>50%），这表明各地点间的基因流动存在阻碍。
• 遗传上不同的种群地点可分为低地、山谷和高地集群；各地点间的遗传相似性随着海拔每100米略有超过4%的差异而降低，从相邻地点间约80%降至海拔差异600米时的50%。
• 结合之前收集的生理数据，分子证据证实野生种群比栽培品种更多样化，并表明从栽培品种到野生种群的基因流动很少，这可能是由于生殖不同步以及在已建立的种群中实生苗补充机会很少所致。各地点间遗传分化的确切原因尚不清楚，需要进一步研究。
