Bala Babul, Mallik Mithun, Saclain Saidin, Islam Md Shahidul
Department of Biotechnology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.
J Genet Eng Biotechnol. 2017 Jun;15(1):23-30. doi: 10.1016/j.jgeb.2017.02.006. Epub 2017 Mar 18.
Sustainable improvement and conservation of any genetic resource depend on the assessment of its intra- and inter-population genetic variation. In order to estimate genetic variation in both wild and hatchery populations of , randomly amplified polymorphic DNA (RAPD) analysis was performed. Analyses of 51 polymorphic loci amplified from genomic DNA by three decamer random primers revealed different degrees of genetic variation in two wild (Bhairab and Rupsha rivers) and hatchery-derived (-1 and -2) populations. The proportion of polymorphic loci was found to be higher in wild populations (0.90 and 0.65 for the Bhairab and Rupsha populations, respectively) than the hatchery-derived populations (0.29 and 0.16 for -1 and -2, respectively). Likewise, the river populations contained higher levels of gene diversity (0.221 and 0.179 for Bhairab and Rupsha populations, respectively) than the populations (0.114 and 0.045 for -1 and -2, respectively). These results suggest reduction of genetic variation and heterozygosity in the hatchery-derived populations. Inter-population similarity indices and pairwise genetic distance values showed that variation between the wild or between the populations were lower than those between the wild and hatchery populations. On average, 14 loci exhibited significant deviation from homogeneity in wild vs hatchery population pairs, whereas 2 and 3 loci showed heterogeneity in -1 vs -2 and Bhairab vs Rupsha population pairs, respectively. A genetic distance-based UPGMA dendrogram segregated river populations from the populations. Our study, therefore, revealed substantial levels of genetic variation between wild and hatchery populations of .
任何遗传资源的可持续改良和保护都依赖于对其种群内和种群间遗传变异的评估。为了估计[具体物种]野生种群和孵化场种群的遗传变异，进行了随机扩增多态性DNA（RAPD）分析。用三种十聚体随机引物从基因组DNA中扩增出51个多态性位点，分析显示两个野生种群（Bhairab河和Rupsha河）以及孵化场来源的[具体物种]种群（-1和-2）存在不同程度的遗传变异。发现野生种群中多态性位点的比例（Bhairab种群和Rupsha种群分别为0.90和0.65）高于孵化场来源的[具体物种]种群（-1和-2分别为0.29和0.16）。同样，河流种群的基因多样性水平（Bhairab种群和Rupsha种群分别为0.221和0.179）高于[具体物种]种群（-1和-2分别为0.114和0.045）。这些结果表明孵化场来源的[具体物种]种群的遗传变异和杂合性有所降低。种群间相似性指数和成对遗传距离值表明，野生种群之间或[具体物种]种群之间的变异低于野生种群与孵化场种群之间的变异。平均而言，14个位点在野生种群与孵化场种群对中表现出显著的偏离同质性，而在-1与-2种群对以及Bhairab与Rupsha种群对中分别有2个和3个位点表现出异质性。基于遗传距离的UPGMA树状图将河流种群与[具体物种]种群区分开来。因此，我们的研究揭示了[具体物种]野生种群和孵化场种群之间存在大量的遗传变异。
