Tsai Chung-Jui, Hubscher Sandra L
Plant Biotechnology Research Center, School of Forest Resources and Environmental Science, Michigan Technological University, Houghton, MI 49931, USA.
New Phytol. 2004 Oct;164(1):73-81. doi: 10.1111/j.1469-8137.2004.01150.x.
The release of the first tree genome sequence for Populus tricocarpa, along with continuing rapid development of Populus EST, microarray and metabolomic technologies, promises to advance forest tree genomics. High throughput transformation by knockout or other gene manipulation strategies to generate large numbers of transgenic Populus for functional elucidation of the estimated 36 000 genes will soon follow. The efficacy of large-scale transformation approaches for advancing Populus genomics will ultimately depend on the availability of properly maintained transgenic germ lines for distribution. The Arabidopsis community has already demonstrated the value of shared genetic resources, especially mutant and transgenic seed stocks, in functional genomics research. Seed bank will not work for the Populus research community. Instead, a stock distribution center for transgenic Populus research will have to be based on long-term preservation of clonal germplasm. This review presents recent progress in applying cryogenic techniques for clonal preservation of long-lived trees and discusses the challenges facing Populus molecular geneticists in germplasm preservation.
毛果杨首个树木基因组序列的公布，以及杨树EST、微阵列和代谢组学技术的持续快速发展，有望推动林木基因组学的进步。通过敲除或其他基因操作策略进行高通量转化，以产生大量转基因杨树用于阐明估计的36000个基因的功能，这将很快实现。大规模转化方法推进杨树基因组学的成效最终将取决于是否有妥善保存的转基因种质系可供分发。拟南芥研究群体已经证明了共享遗传资源，特别是突变体和转基因种子库在功能基因组学研究中的价值。种子库不适用于杨树研究群体。相反，杨树转基因研究的种质分发中心必须基于长期保存克隆种质。本综述介绍了应用低温技术对长寿树木进行克隆保存的最新进展，并讨论了杨树分子遗传学家在种质保存方面面临的挑战。
