Bridge Paul D, Roberts Peter J, Spooner Brian M, Panchal Gita
School of Biological and Chemical Sciences, Birkbeck College, Malet Street, London WC1E 7HX, UK.
Mycology Section, Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3AB, UK.
New Phytol. 2003 Oct;160(1):43-48. doi: 10.1046/j.1469-8137.2003.00861.x.
• Here, the reliability of published fungal nucleic acid sequences is tested by the critical re-evaluation of 206 named sequences obtained from public-access databases. • Sequences from the ribosomal RNA (rRNA) gene cluster were examined as these are commonly used to establish fungal phylogeny and evolution, and are also increasingly employed in the identification of fungi from nonculture based studies. • Fifty-one rRNA internal transcribed spacer (ITS) sequences were obtained for species of Amanita, 55 ITS sequences were obtained for species of Phoma and 100 rRNA small subunit sequences were obtained from representative genera of the order Helotiales. In each case, the fungal group was selected partly on the basis of sequences deposited by three or more laboratories in order to avoid sample bias. The results suggest that up to 20% of the sequences available for each group may be unreliable, and this proportion is supported by additional informal observations.
• 在此，通过对从公共访问数据库中获取的206个命名序列进行严格的重新评估，来测试已发表的真菌核酸序列的可靠性。• 对核糖体RNA（rRNA）基因簇的序列进行了检查，因为这些序列通常用于建立真菌的系统发育和进化关系，并且在基于非培养研究的真菌鉴定中也越来越多地被采用。• 获得了51个鹅膏菌属物种的rRNA内部转录间隔区（ITS）序列，55个茎点霉属物种的ITS序列，以及从柔膜菌目代表性属中获得的100个rRNA小亚基序列。在每种情况下，真菌类群的选择部分基于三个或更多实验室所保存的序列，以避免样本偏差。结果表明，每组中高达20%的可用序列可能不可靠，这一比例得到了其他非正式观察结果的支持。
