Larcher M, Muller B, Mantelin S, Rapior S, Cleyet-Marel J-C
Laboratoire des Symbioses Tropicales et Méditerranéennes, INRA, IRD, CIRAD, AGRO-M, UM2, Campus de Baillarguet, TA 10/J, F-34398 Montpellier Cedex 5 France.
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, INRA, AGRO-M, Place Viala, F-34060 Montpellier cedex 2 France.
New Phytol. 2003 Oct;160(1):119-125. doi: 10.1046/j.1469-8137.2003.00862.x.
• Plant growth-promoting bacteria (PGPB) have been reported to stimulate root morphogenesis. To improve our knowledge of the PGPB effect, the early modifications of Brassica napus root system architecture induced by the PGPB Phyllobacterium sp. (29-15) were analysed. • Plants were grown in Petri dishes on a vertical medium supplemented with variable doses of Phyllobacterium sp. in gnotobiotic conditions. Root system elementary variables were measured in a nondestructive manner and the distribution of the bacteria throughout the primary root was quantified. • Phyllobacterium sp. in doses from 3 × 10 to 3 × 10 colony-forming units ml significantly promoted B. napus total root length up to 50% by increasing both lateral root density throughout the primary root and growth rate of mature lateral roots. The primary root was progressively colonized by the bacteria from the tip to the base and the number of colonizing cells was positively correlated with the inoculum density. • Relationships between inoculum density, root colonization and root system architecture emphasized the relevance of this approach to specify PGPB effects on plants.
• 据报道，植物促生细菌（PGPB）可刺激根系形态发生。为了增进我们对PGPB作用的了解，对PGPB类芽孢杆菌属（29-15）诱导的甘蓝型油菜根系结构早期变化进行了分析。
• 将植物种植在培养皿中的垂直培养基上，在无菌条件下添加不同剂量的类芽孢杆菌属。以无损方式测量根系基本变量，并对细菌在整个主根中的分布进行定量。
• 剂量为3×10至3×10菌落形成单位/毫升的类芽孢杆菌属通过增加整个主根的侧根密度和成熟侧根的生长速率，显著促进甘蓝型油菜总根长增长达50%。细菌从根尖到基部逐渐定殖于主根，定殖细胞数量与接种密度呈正相关。
• 接种密度、根定殖与根系结构之间的关系强调了这种方法对于明确PGPB对植物作用的相关性。
