Zhao Hongcheng, Sun Nan, Huang Lin, Qian Ruyi, Lin Xianyong, Sun Chengliang, Zhu Yongguan
MOE Key Laboratory of Environment Remediation and Ecological Health, College of Natural Resource & Environmental Sciences, Zhejiang University, Hangzhou 310058, China.
Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, PR China.
iScience. 2023 Jun 15;26(7):107144. doi: 10.1016/j.isci.2023.107144. eCollection 2023 Jul 21.
The molecular mechanism of beneficial bacterium -mediated root developmental remain elusive. elicited extensively transcriptional changes but inhibited primary root elongation in . By analyzing root cell type-specific developmental markers, we demonstrated that affected neither overall organization nor cell division of primary root meristem. The cessation of primary root resulted from reduction of cell elongation, which is probably because of bacterially activated peroxidase that will lead to cell wall cross-linking at consuming of HO. The activated peroxidase combined with downregulated cell wall loosening enzymes consequently led to cell wall thickness, whereas inhibiting peroxidase restored root growth under inoculation. We further showed that peroxidase activity was probably promoted by cadaverine secreted by . These results suggest that inhibits root elongation by activating peroxidase and inducing cell wall modification in , in which cadaverine released by is a potential signal compound.
有益细菌介导根系发育的分子机制仍不清楚。在[具体植物]中引发了广泛的转录变化，但抑制了主根伸长。通过分析根细胞类型特异性发育标记，我们证明[有益细菌]既不影响主根分生组织的整体组织也不影响其细胞分裂。主根生长的停止是由于细胞伸长减少，这可能是因为细菌激活的过氧化物酶在消耗过氧化氢时会导致细胞壁交联。激活的过氧化物酶与下调的细胞壁松弛酶相结合，从而导致细胞壁增厚，而抑制过氧化物酶可在[有益细菌]接种条件下恢复根的生长。我们进一步表明，过氧化物酶活性可能是由[有益细菌]分泌的尸胺促进的。这些结果表明，[有益细菌]通过激活过氧化物酶和诱导[具体植物]中的细胞壁修饰来抑制根伸长，其中[有益细菌]释放的尸胺是一种潜在的信号化合物。
