Rosling A, Landeweert R, Lindahl B D, Larsson K-H, Kuyper T W, Taylor A F S, Finlay R D
Department of Forest Mycology and Pathology, SLU, Box 7026, 750 07 Uppsala, Sweden.
Sub-Department of Soil Quality, Wageningen University, Box 8005, NL-6700 EC, Wageningen, The Netherlands.
New Phytol. 2003 Sep;159(3):775-783. doi: 10.1046/j.1469-8137.2003.00829.x.
• Studies of ectomycorrhizal fungal communities in forest soils are usually restricted to the uppermost organic horizons. Boreal forest podzols are highly stratified and little is known about the vertical distribution of ectomycorrhizal communities in the underlying mineral horizons. • Ectomycorrhizal root tips were sampled from seven horizons in three continuous columns of a 52-cm deep podzol profile. Root tips were sorted into morphological groups and the colonising fungi identified by sequencing of the rDNA ITS region. The vertical distribution of mycorrhizal taxa was examined. • A relationship between ectomycorrhizal species composition and soil horizon was found. Tomentellopsis submollis, three Piloderma species and Dermocybe spp. were found predominantly in the upper horizons while Suillus luteus, Lactarius utilis and three undescribed Piloderma species were associated with the mineral horizons. • Two thirds of the root tips were found in the mineral soil and half of the taxa were restricted to the mineral horizons. The results highlight the need to include the mineral soil in order to gain a more accurate representation of the ectomycorrhizal community.
• 森林土壤中外生菌根真菌群落的研究通常局限于最上层的有机层。北方森林灰化土分层明显，对于下层矿质层中外生菌根群落的垂直分布了解甚少。
• 从一个52厘米深的灰化土剖面的三个连续柱状样的七个土层中采集外生菌根根尖。将根尖分类为形态学组，并通过rDNA ITS区域测序鉴定定殖真菌。研究了菌根类群的垂直分布。
• 发现外生菌根物种组成与土壤层之间存在关系。软毛拟丝膜菌、三种皮盘菌属真菌和皮伞属真菌主要出现在上层，而褐环乳牛肝菌、乳白乳菇和三种未描述的皮盘菌属真菌与矿质层有关。
• 三分之二的根尖发现于矿质土中，一半的类群局限于矿质层。结果强调需要纳入矿质土，以便更准确地呈现外生菌根群落。
