Stanek Małgorzata, Kushwaha Priyanka, Murawska-Wlodarczyk Kamila, Stefanowicz Anna M, Babst-Kostecka Alicja
W. Szafer Institute of Botany, Polish Academy of Sciences, Lubicz 46, 31-512 Kraków, Poland.
Department of Environmental Science, The University of Arizona, Tucson, AZ 85721, USA.
Geoderma. 2023 Feb;430. doi: 10.1016/j.geoderma.2023.116328. Epub 2023 Jan 11.
Invasive plants can modify the diversity and taxonomical structure of soil microbiomes. However, it is difficult to generalize the underlying factors as their influence often seems to depend on the complex plant-soil-microbial interactions. In this paper, we investigated how impacts on the soil microbiome across two soil horizons in relation to native woodland. Five paired adjacent invaded vs native vegetation plots in a managed forest in southern Poland were investigated. Soil microbial communities were assessed along with soil enzyme activities and soil physicochemical parameters, separately for both organic and mineral horizons, as well as forest stand characteristics to explore plant-soil-microbe interactions. Although did not significantly affect pH, organic C, total N, available nutrients nor enzymatic activity, differences in soil abiotic properties (except C to N ratio) were primarily driven by soil depth for both vegetation types. Further, we found significant differences in soil microbiome under invasion in relation to native vegetation. Microbial richness and diversity were lower in both horizons of vs control plots. Moreover, increased relative abundance of unique amplicon sequence variants in both horizons and thereby significantly changed the structure of the core soil microbial communities, in comparison to the control plots. In addition, predicted microbial functional groups indicated a predominant soil depth effect in both vegetation plots with higher abundance of aerobic chemoheterotrophic bacteria and endophytic fungi in the organic horizon and greater abundance of methanotrophic and methylotrophic bacteria, and ectomycorrhizal fungi in the mineral horizon. Overall, our results indicate strong associations between invasion and changes in soil microbiome and associated functions, a finding that needs to be further investigated to predict modifications in ecosystem functioning caused by this invasive species.
入侵植物能够改变土壤微生物群落的多样性和分类结构。然而，由于其影响似乎常常取决于复杂的植物-土壤-微生物相互作用，因此很难归纳出潜在因素。在本文中，我们研究了入侵植物对与原生林地相关的两个土壤层中土壤微生物群落的影响。我们调查了波兰南部一处人工林中五对相邻的入侵植被与原生植被地块。分别针对有机层和矿质层，对土壤微生物群落、土壤酶活性以及土壤理化参数进行了评估，并结合林分特征来探究植物-土壤-微生物之间的相互作用。尽管入侵植物对土壤pH值、有机碳、全氮、有效养分以及酶活性没有显著影响，但对于这两种植被类型而言，土壤非生物性质（除碳氮比外）的差异主要由土壤深度驱动。此外，我们发现入侵植物下的土壤微生物群落与原生植被相比存在显著差异。与对照地块相比，入侵植物地块两个土层中的微生物丰富度和多样性均较低。此外，入侵植物增加了两个土层中独特扩增子序列变体的相对丰度，从而显著改变了核心土壤微生物群落的结构。此外，预测的微生物功能组表明，在这两种植被地块中，土壤深度效应均占主导地位，在有机层中需氧化学异养细菌和内生真菌的丰度较高，而在矿质层中甲烷营养菌和甲基营养菌以及外生菌根真菌的丰度较高。总体而言，我们的结果表明入侵植物与土壤微生物群落及其相关功能的变化之间存在紧密联系，这一发现需要进一步研究，以预测该入侵物种对生态系统功能的改变。
