Watts S H, Wheeler C T, Hillman J R
Department of Botany, University of Glasgow, Glasgow G12 8QQ, UK.
New Phytol. 1987 Mar;105(3):459-468. doi: 10.1111/j.1469-8137.1987.tb00883.x.
Treatment of three-month or six-month-old water culture plants of Alnus glutinosa with 0.1 mol m (±) abscisic acid (ABA) induced formation of resting buds after 30 to 60 d. Leaf dry weight per plant decreased by 40 to 45 % during this period but root and nodule dry weights were unchanged relative to control plants. [2- C] ABA was taken up by the root system within 5 d and after 30 or 60 d the ABA content of plants fed 01 mol m ABA was 60 and 119 times that of control plants. In control plants, the levels of 'free'cis(Z) ABA were similar to or exceeded 'free'trans(E) ABA in all plant parts while, in general, the converse was true for these isomers in 'bound' form. In ABA-treated plants, all isomeric forms of ABA were present at much higher amounts than in the controls but, whereas in leaves and shoot apices the amount of cis ABA in both 'free' and 'bound' forms was much greater than the trans isomer, in roots and nodules trans ABA was the dominant isomer in both 'bound' and 'free' forms. Most biologically active 'free'cis ABA thus accumulated in the leaves and shoot apex, where effects of ABA treatment on growth were most evident, whereas in roots and nodules where biologically inactive 'free' and 'bound'trans ABA were dominant, growth was relatively unaffected. Amounts of 'free' ABA in nodules were several times those recorded previously in nodules of dormant plants, in keeping with earlier suggestions that ABA is unlikely to have a precise regulatory role in growth and dormancy of nodules.
用0.1 mol m (±)脱落酸（ABA）处理三个月或六个月大的水培欧洲桤木植株，30至60天后诱导形成休眠芽。在此期间，单株叶片干重下降了40%至45%，但根和根瘤的干重相对于对照植株没有变化。[2- C] ABA在5天内被根系吸收，30或60天后，用01 mol m ABA处理的植株的ABA含量分别是对照植株的60倍和119倍。在对照植株中，所有植物部位的“游离”顺式（Z）ABA水平与“游离”反式（E）ABA相似或超过后者，而一般来说，这些异构体在“结合”形式下情况相反。在ABA处理的植株中，ABA的所有异构体形式的含量都比对照植株高得多，但是，在叶片和茎尖中，“游离”和“结合”形式的顺式ABA含量都远大于反式异构体，而在根和根瘤中，反式ABA在“结合”和“游离”形式中都是主要异构体。因此，大多数具有生物活性的“游离”顺式ABA积累在叶片和茎尖，ABA处理对生长的影响在这些部位最为明显，而在根和根瘤中，无生物活性的“游离”和“结合”反式ABA占主导，生长相对未受影响。根瘤中“游离”ABA的含量是先前在休眠植物根瘤中记录值的几倍，这与早期认为ABA不太可能在根瘤的生长和休眠中起精确调节作用的观点一致。
