Debaud J C, Gay G
Université Claude Bernard LYON I, Institut de Chimie et de Biologie Moléculaires et Cellulaires, Unité de Mycologie associée au CNRS 1127 Bât. 405, 43 Boulevard du 11 Novembre, F 69622 Villeurbanne, Cedex, France.
New Phytol. 1987 Mar;105(3):429-435. doi: 10.1111/j.1469-8137.1987.tb00880.x.
The ectomycorrhizal fungus Hebeloma cylindrosporum Romagnési produced sporulating fruit bodies under axenic conditions on a synthetic medium when cultivated with its usual host plant Pinus pinaster (Sol.). In the absence of the host plant, H. cylindrosporum only formed primordia or immature fruit bodies. In 11 Erlenmeyer flasks, the fungal fruiting started about one and half months after the inoculation of one-month-old P. pinaster plants by the mycelium of H. cylindrosporum, and a total of 144 fruit bodies appeared in 45 flasks during a 9.5-month fruiting period. At 18 °C, the mean number of basidiomes developed per flask was about 3-5. A thermal shock of ± 6 °C for 5 d did not significantly affect the mean number of basidiomes per flask but modified the time course of their rate of formation. Spores were collected from the fruit bodies and were found to be capable of germinating.
外生菌根真菌柱孢疣柄牛肝菌（Hebeloma cylindrosporum Romagnési）在无菌条件下，于合成培养基上与它的常见寄主植物海岸松（Pinus pinaster (Sol.)）共同培养时，会产生形成孢子的子实体。在没有寄主植物的情况下，柱孢疣柄牛肝菌仅形成原基或未成熟的子实体。在11个锥形瓶中，用柱孢疣柄牛肝菌的菌丝体接种1月龄的海岸松植株约一个半月后，真菌开始结实，在9.5个月的结实期内，45个锥形瓶中共出现了144个子实体。在18℃时，每个锥形瓶发育出的担子果平均数量约为3 - 5个。±6℃的热激处理5天，对每个锥形瓶担子果的平均数量没有显著影响，但改变了其形成速率的时间进程。从子实体上收集的孢子能够萌发。
