Debaud J C, Gay G, Prevost A, Lei J, Dexheimer J
Laboratoire de Mycologie associe an CNRS, Institut de Chime et de Biologie Moléculaires et Cellulaires, Université Lyon 1, 43 Boulevard du 11 Novembre, 69622 Villeurbanne Cedex France.
Laboratoire de Biologie des Ligneux, J.E. CNRS 034613, Université Nancy 1, B.P. 239, 54506 Vandoeuvre Cedex France.
New Phytol. 1988 Mar;108(3):323-328. doi: 10.1111/j.1469-8137.1988.tb04169.x.
Ectomycorrhizas were synthesized under axenic conditions between Pinus pinaster (Ait.) Sol. and different mycelial cultures of Hebeloma cylindrosporum Romagnesi. These included a wild dikaryon and four sib-homokaryons (one for each mating type) belonging to the progeny of this fruiting strain. The homokaryotic mycelia formed typical ectomyeorrhizas showing a morphology and an ultrastructural organization similar to that of ectomyeorrhizas obtained with the parental dikaryon. The ultrastructural localization of acid phosphatase activities was also comparable for homokaryotic and dikaryotic mycorrhizas. These results demonstrate that the ectomycorrhizal ability is not restricted to the dikaryotic state of fungal life-cycle since homokaryotic mycelia also formed typical and functional ectomyeorrhizas. The formation of normal mycorrhizas by homokaryotic mycelia confirms the usefulness of H. cylindrosporum as a model for genetical studies and improvement of ectomycorrhizal fungi by means of chromosomal genetics.
在无菌条件下，将海岸松（Pinus pinaster (Ait.) Sol.）与圆柱齿梗孢（Hebeloma cylindrosporum Romagnesi）的不同菌丝体培养物合成了外生菌根。这些培养物包括一个野生双核体和四个属于该子实体菌株后代的同核体同胞（每个交配型一个）。同核菌丝体形成了典型的外生菌根，其形态和超微结构组织与亲本双核体形成的外生菌根相似。同核和双核菌根的酸性磷酸酶活性的超微结构定位也具有可比性。这些结果表明，外生菌根形成能力并不局限于真菌生命周期的双核状态，因为同核菌丝体也能形成典型且功能正常的外生菌根。同核菌丝体形成正常菌根这一现象证实了圆柱齿梗孢作为通过染色体遗传学进行遗传研究和改良外生菌根真菌模型的实用性。
