Gordon A J, Thomas B J, Reynolds P H S
AFRC Institute of Grassland and Environmental Research, Welsh Plant Breeding Station, Plas Gogerddan, Aberystwyth, Dyfed SY23 3EB.
Biotechnology Division, DSIR, Palmerston North, New Zealand.
New Phytol. 1992 Sep;122(1):35-44. doi: 10.1111/j.1469-8137.1992.tb00050.x.
Immunogold labelling studies using polyclonal antibodies to sucrose synthase suggest that this enzyme is exclusively located in the cytoplasm of soybean nodule cells. A significantly greater intensity of labelling was found in the cytosol of uninfected interstitial cells of the central nodule region compared with the cytosol of the infected cells. Labelling was low in the bulk of the cortical cells but was found to be high in the vascular endodermis and in the cortical cells between the bundle and the infected region. The results are discussed in relation to carbohydrate supply and metabolism and in the context of nodule structure.
使用抗蔗糖合酶多克隆抗体的免疫金标记研究表明，这种酶仅位于大豆根瘤细胞的细胞质中。与受感染细胞的胞质溶胶相比，在中央根瘤区域未受感染的间质细胞的胞质溶胶中发现标记强度明显更高。在大部分皮层细胞中标记较低，但在维管束内皮层以及维管束与受感染区域之间的皮层细胞中发现标记较高。结合碳水化合物供应和代谢以及根瘤结构的背景对结果进行了讨论。
