Tonkin C M, Malajczuk N, McCOMB J A
School of Environmental and Life Sciences, Murdoch University, Murdoch, Western Australia 6150.
*Division of Forest Research CSIRO, Private Bag, P.O. Wembley, Western Australia 6014.
New Phytol. 1989 Feb;111(2):209-214. doi: 10.1111/j.1469-8137.1989.tb00684.x.
Eucalyptus marginata Donn ex Sm. and Pisolithus tinctorius (Pers.) Cok and Couch were co-cultured to obtain ectomycorrhizal formation in vitro. One isolate of P. tinctorius formed mycorrhizas with aseptic seedlings of a juvenile clone derived from a 4-month-old seedling, and four clones derived from crowns of mature trees. A second P. tinctorius isolate formed mycorrhizas with only the clones from mature trees. Successful combinations resulted in formation of a mantle followed by a Hartig net and epidermal cell elongation. The fungal/seedlings or fungal/seedling clone combinations which did not produce ectomycorrhizal roots, were characterized by a mantle but lacked a Hartig net, and formed an abundance of polyphenols throughout the root. Genotype, maturity and fungal specificity are key factors influencing successful ectomycorrhizal formation on E. marginata by P. tinctorius in vitro.
将边缘桉（Eucalyptus marginata Donn ex Sm.）与彩色豆马勃（Pisolithus tinctorius (Pers.) Cok and Couch）进行共培养，以在体外获得外生菌根的形成。一株彩色豆马勃与一株源自4个月大幼苗的幼年无性系无菌苗以及四株源自成熟树冠的无性系形成了菌根。另一株彩色豆马勃仅与源自成熟树的无性系形成了菌根。成功的组合会导致形成菌套，随后形成哈蒂氏网以及表皮细胞伸长。未产生外生菌根根的真菌/幼苗或真菌/幼苗无性系组合，其特征是有菌套但没有哈蒂氏网，并且在整个根中形成大量多酚。基因型、成熟度和真菌特异性是影响彩色豆马勃在体外成功与边缘桉形成外生菌根的关键因素。
