Ludwig-Müller Jutta, Bendel Ulrike, Thermann Petra, Ruppel Manfred, Epstein Ephraim, Hilgenberg Willy
Botanisches Institut, J. W. Goethe-Universität, Siesmayerstr. 70, 60054 Frankfurt am Main, Germany.
Department of Horticulture, Agricultural Research Organization, The Volcani Center, Bet Dagan 50 250, Israel.
New Phytol. 1993 Dec;125(4):763-769. doi: 10.1111/j.1469-8137.1993.tb03926.x.
A convenient method for infecting Brassica seedlings in liquid medium with the Phytopathogenic fungus Plasmodiophora brassicae was developed. The infection rates of two susceptible and two tolerant Chinese cabbage varieties with wild type P. brassicae were investigated. The content of free indole-3-acetic acid (IAA) was determined using combined gas chromatography-mass spectrometry (GC-MS) with [ C ]-IAA as internal standard during the first period of infection with the fungus (5-14 d) and compared with the indole glucosinolate content of two Chinese cabbage varieties (one tolerant, one susceptible) in the same developmental stages, The results showed that the mean of the IAA content in the infected plants was approximately 66.5% higher than that of the non-infected controls. In both the susceptible and tolerant varieties higher levels of IAA were found in the infected plants 10 d after the beginning of incubation. After 14 d of incubation IAA levels decreased in the susceptible, infected plants and increased in the tolerant, infected plants.
开发了一种在液体培养基中用致病真菌芸薹根肿菌感染甘蓝型油菜幼苗的简便方法。研究了两个易感和两个耐病大白菜品种对野生型芸薹根肿菌的感染率。在真菌感染的第一阶段（5 - 14天），以[ C ] - 吲哚 - 3 - 乙酸（IAA）作为内标，采用气相色谱 - 质谱联用（GC - MS）法测定游离吲哚 - 3 - 乙酸（IAA）的含量，并与两个处于相同发育阶段的大白菜品种（一个耐病，一个易感）的吲哚硫苷含量进行比较。结果表明，受感染植株中IAA含量的平均值比未受感染的对照植株高出约66.5%。在易感和耐病品种中，培养开始10天后，受感染植株中的IAA水平均较高。培养14天后，易感的受感染植株中IAA水平下降，而耐病的受感染植株中IAA水平上升。
