基因在受[病原体名称]感染的大白菜中的作用分析。（注：原文中“.”处应补充具体病原体名称）

Analysis of the role of gene in Chinese cabbage infected by .

作者信息

Ge Wenjie, Lv Mingcan, Feng Hui, Wang Xinlei, Zhang Bo, Li Ken, Zhang Jing, Zou Jiawei, Ji Ruiqin

机构信息

Liaoning Key Laboratory of Genetics and Breeding for Cruciferous Vegetable Crops, College of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning, China.

出版信息

Front Plant Sci. 2023 Jan 25;14:1082395. doi: 10.3389/fpls.2023.1082395. eCollection 2023.

DOI:10.3389/fpls.2023.1082395

PMID:36760653

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9905630/

Abstract

INTRODUCTION

The clubroot disease caused by () poses a serious threat to the economic value of cruciferous crops, which is a serious problem to be solved worldwide. Some resistance genes to clubroot disease in L. ssp cause by have been located on different chromosomes. Among them, and were mapped to the common candidate gene , but its resistance mechanism is not clear yet.

METHODS

In this experiment, the differences of between the resistant and susceptible material of Chinese cabbage were analyzed by gene cloning and qRT-PCR. The gene function was verified by Arabidopsis homologous mutants. The expression site of gene in cells was analyzed by subcellular localization. Finally, the candidate interaction protein of was screened by yeast two-hybrid library.

RESULTS

The results showed that the cDNA sequence, upstream promoter sequence and expression level of were quite different between the resistant and susceptible material. The resistance investigation found that the Arabidopsis mutant was more susceptible to clubroot disease than the wild type, which suggested that the deletion of rpp1 reduces resistance of plant to clubroot disease. Subcellular location analysis confirmed that was located in the nucleus. The interaction proteins of screened from cDNA Yeast Library by yeast two-hybrid are mainly related to photosynthesis, cell wall modification, jasmonic acid signal transduction and programmed cell death.

DISCUSSION

gene contains TIR-NBS-LRR domain and belongs to R gene. The cDNA and promoter sequence of BrRPP1 in resistant varieties was different from that in susceptible varieties led to the significant difference of the gene expression of between the resistant varieties and the susceptible varieties. The high expression of gene in resistant varieties enhanced the resistance of Chinese cabbage to , and the interaction proteins of are mainly related to photosynthesis, cell wall modification, jasmonic acid signal transduction and programmed cell death. These results provide important clues for understanding the mechanism of BrRPP1 in the resistance of B. rapa to .

摘要

引言

由（）引起的根肿病对十字花科作物的经济价值构成严重威胁，这是一个在全球范围内亟待解决的严重问题。一些由（）引起的甘蓝型油菜根肿病抗性基因已定位在不同染色体上。其中，（）和（）被定位到共同的候选基因（），但其抗性机制尚不清楚。

方法

本实验通过基因克隆和qRT-PCR分析了大白菜抗感材料之间（）的差异。通过拟南芥同源突变体验证基因功能。通过亚细胞定位分析（）基因在细胞中的表达位点。最后，通过酵母双杂交文库筛选（）的候选互作蛋白。

结果

结果表明，抗感材料之间（）的cDNA序列、上游启动子序列和表达水平存在较大差异。抗性调查发现，拟南芥突变体（）比野生型更易感染根肿病，这表明rpp1的缺失降低了植物对根肿病的抗性。亚细胞定位分析证实（）位于细胞核中。通过酵母双杂交从cDNA酵母文库中筛选出的（）互作蛋白主要与光合作用、细胞壁修饰、茉莉酸信号转导和程序性细胞死亡有关。

讨论

（）基因含有TIR-NBS-LRR结构域，属于R基因。抗性品种中BrRPP1的cDNA和启动子序列与感病品种不同，导致抗感品种之间（）基因表达存在显著差异。（）基因在抗性品种中的高表达增强了大白菜对（）的抗性，（）的互作蛋白主要与光合作用、细胞壁修饰、茉莉酸信号转导和程序性细胞死亡有关。这些结果为理解BrRPP1在白菜对（）抗性中的作用机制提供了重要线索。