Griffith G W, Hedger J N
Institute of Biological Sciences, University of Wales, Abervstwyth, Dyfed, SY23 3D A, Wales, UK.
New Phytol. 1994 Jun;127(2):243-259. doi: 10.1111/j.1469-8137.1994.tb04276.x.
A detailed investigation of the Liana biotype (L-biotype) of Crinipellis perniciosa (Stahel) Sing, was conducted in Ecuador. Basidiocarps of the L-biotype were consistently found in association with stems or debris of the liana Arrabidaea verrucosa (Standl) A. Gentry, although no symptoms associated with witches'broom disease, were observed. Debris colonized by the L-biotype were often connected via pseudosclerotial pads to living sterns of A. verrucosa. Pairings between 87 isolates obtained from basidiocarp stipe tissues or liana bark cores from three sites permitted 37 somatic compatibility groupings (SCGs) to be defined. In all cases these were found to be very restricted in geographical distribution, a situation which contrasts markedly with the distribution of SCGs in the pathogenic and non-outercrossing eocoa biotype. Mating pairings between single basidiospore isolates confirmed chat the outercrossing mechanism of the L-biotype is muitialletic and that no significant biological barriers to gene flow exist between coastal and Amazonian populations. Several lines of evidence suggested that mycelia belonging to the same SCG (= genet) were isogenic. Correlations between field observations and the distributions of both SCGs and mating type factors art- discussed in relation to patterns of dispersal, establishment and spread of this fungus.
在厄瓜多尔对有害红褶菌（Crinipellis perniciosa (Stahel) Sing）的藤本生物型（L-生物型）进行了详细调查。尽管未观察到与丛枝病相关的症状，但始终发现L-生物型的担子果与藤本植物疣枝节柄木（Arrabidaea verrucosa (Standl) A. Gentry）的茎或残骸相关联。被L-生物型定殖的残骸通常通过假菌核垫与疣枝节柄木的活茎相连。从三个地点的担子果柄组织或藤本植物树皮芯中获得的87个分离株之间的配对，确定了37个体细胞相容性组（SCGs）。在所有情况下，发现这些组在地理分布上非常有限，这种情况与致病和非异交可可生物型中SCGs的分布形成明显对比。单个担孢子分离株之间的交配配对证实，L-生物型的异交机制是多等位基因的，并且沿海和亚马逊种群之间不存在对基因流动的重大生物学障碍。几条证据表明，属于同一SCG（=基因）的菌丝体是同基因的。结合这种真菌的传播、定殖和扩散模式，讨论了实地观察与SCGs分布和交配型因子之间的相关性。
