Filho Dorival F, Pungartnik Cristina, Cascardo Julio C M, Brendel Martin
Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna, Km 16, Ilhéus, BA, CEP 45650WW-000, Brasil.
Curr Microbiol. 2006 May;52(5):407-12. doi: 10.1007/s00284-005-0405-3. Epub 2006 Apr 1.
Breaking of hyphae derived from growth of the phytopathogenic fungus Crinipellis perniciosa in liquid media yielded cell aggregates that performed as "quasi single cell" in toxicity assays. When treated with the chemical mutagens 4-nitroquinoleine-1-oxide (4NQO), hydrogen peroxide (H2O2), or paraquat (PAQ) as well as with ultraviolet light (UVC), broken hyphae of C. perniciosa gave a single cell-like response, i.e., survival curves similar to those obtained when treating single-cell suspensions. C. perniciosa had significantly higher UVC resistance than haploid bakers yeast but was more sensitive to 4NQO and extremely sensitive to PAQ and H2O2 when compared to likewise-treated yeast. Haploid C. perniciosa basidiospores (monokaryotic) were significantly more UVC resistant than C. perniciosa broken hyphae and than haploid and diploid yeast wild-type strains. This suggests a high capacity in C. perniciosa for repair of UVC-induced DNA lesions or, alternatively, an efficient protection from UVC irradiation, especially in basidiospores. The pronounced sensitivity of the dikaryotic form of C. perniciosa to PAQ and H2O2 points to a weak protection from oxidative stress-inducing agents.
从植物病原真菌有害红褶菌（Crinipellis perniciosa）在液体培养基中的生长所衍生的菌丝断裂，产生了在毒性试验中表现为“准单细胞”的细胞聚集体。当用化学诱变剂4-硝基喹啉-1-氧化物（4NQO）、过氧化氢（H2O2）或百草枯（PAQ）以及紫外线（UVC）处理时，有害红褶菌的断裂菌丝给出了类似单细胞的反应，即存活曲线类似于处理单细胞悬浮液时获得的曲线。与同样处理的酵母相比，有害红褶菌对UVC的抗性明显高于单倍体面包酵母，但对4NQO更敏感，对PAQ和H2O2极其敏感。单倍体有害红褶菌担孢子（单核）对UVC的抗性明显高于有害红褶菌断裂菌丝以及单倍体和二倍体酵母野生型菌株。这表明有害红褶菌具有很高的修复UVC诱导的DNA损伤的能力，或者，也可能是对UVC辐射有高效的保护作用，尤其是在担孢子中。有害红褶菌双核体形式对PAQ和H2O2的显著敏感性表明其对氧化应激诱导剂的保护较弱。
