Hagen C, Braune W, Birckner E, Nuske J
Institute of General Botany, Friedrich Schiller University Jena, von Hase-Weg 3, D-07743 Jena, Germany.
Institute of Physical Chemistry, Friedrich Schiller University Jena, Steiger 3, D-07743 Jena, Germany.
New Phytol. 1993 Nov;125(3):625-633. doi: 10.1111/j.1469-8137.1993.tb03912.x.
Alterations of photosynthetic activity were investigated during accumulation of secondary carotenoids in Haematococcus lacustris (Girod) Rostafinski. Using several methods regarding (i) pigment pattern and pigment content (cytophotometry, computer-aided microscopic image analysis, in vivo absorption spectroscopy, high-performance liquid chromatrography) and (ii) photosynthesis (microfluorometry, modulated chlorophyll fluorescence and luminescence detection, O evolution and consumption measurement, 77 K fluorescence spectroscopy) we examined single cells and cell suspensions of the green alga. The results indicate decrease of both the photosystem II activity and the linear electron transport accompanying synthesis of secondary carotenoids. On the other hand, activation of those processes leading to an increased transthylakoid proton gradient could be established during early accumulation period. This is assumed to be an active adaptation of the photosynthetic apparatus to energetic requirements given by secondary carotenoid biosynthesis in H. lacustris.
在湖生血球藻（Girod）Rostafinski积累次生类胡萝卜素的过程中，对其光合活性的变化进行了研究。我们使用了几种方法，包括（i）色素模式和色素含量（细胞光度法、计算机辅助显微图像分析、体内吸收光谱法、高效液相色谱法）以及（ii）光合作用（显微荧光测定法、调制叶绿素荧光和发光检测、氧气释放和消耗测量、77K荧光光谱法），来检测这种绿藻的单细胞和细胞悬浮液。结果表明，在次生类胡萝卜素合成过程中，光系统II活性和线性电子传递均降低。另一方面，在早期积累阶段，可以确定导致类囊体间质子梯度增加的那些过程被激活。这被认为是光合装置对湖生血球藻次生类胡萝卜素生物合成所产生的能量需求的一种积极适应。
