Head of the Proteomics Platform, Luxembourg Institute of Health, Strassen, Luxembourg.
Co-Head of the Quantitative Biology Unit, Proteomics of Cellular Signaling Research Group Luxembourg Institute of Health, Strassen, Luxembourg.
Expert Rev Proteomics. 2021 Feb;18(2):75-82. doi: 10.1080/14789450.2021.1908895. Epub 2021 Apr 19.
The continuous technical improvement in sensitivity and specificity placed mass spectrometry as an alternative method for analyzing clinical samples. In parallel to the rapid development of discovery proteomics, targeted acquisition has been implemented as a complementary option for measuring a small set of proteins with high sensitivity and robustness in a large sample cohort. The combination of trapped ion mobility with a rapid time-of-flight (TOF) mass spectrometer improves the sensitivity even further and triggers the development of prm-PASEF.
This article discusses the development of prm-PASEF and its advantages over the existing targeted and discovery methods for analyzing clinical samples. We are also highlighting the different requirements for the use of prm-PASEF on clinical samples.
prm-PASEF takes advantage of a dual ion-mobility trap enabling highly multiplexed targeted acquisition. It allows the implementation of a short chromatographic separation setup without sacrificing the number of targeted peptides. Analyzing clinical samples by prm-PASEF holds the promise to significantly improve throughput while maintaining sensitivity to detect the selected target proteins.
灵敏度和特异性的不断技术提高使质谱成为分析临床样本的替代方法。与发现蛋白质组学的快速发展并行的是，靶向采集已作为一种补充选择，用于在大样本队列中以高灵敏度和稳健性测量一小部分蛋白质。将俘获离子淌度与快速飞行时间（TOF）质谱仪相结合，可进一步提高灵敏度，并引发 prm-PASEF 的发展。
本文讨论了 prm-PASEF 的发展及其相对于现有针对临床样本的靶向和发现方法的优势。我们还强调了在临床样本中使用 prm-PASEF 的不同要求。
prm-PASEF 利用双重离子淌度阱，实现了高度多重化的靶向采集。它允许实现短的色谱分离设置，而不牺牲靶向肽的数量。通过 prm-PASEF 分析临床样本有望在保持对所选目标蛋白质的灵敏度的同时，显著提高通量。
