CentraleSupélec, Laboratoire de Génie des Procédés et Matériaux, SFR Condorcet FR CNRS 3417, Centre Européen de Biotechnologie et de Bioéconomie (CEBB), Université Paris-Saclay, 51110, Pomacle, France.
CentraleSupélec, Laboratoire de Génie des Procédés et Matériaux, Université Paris-Saclay, 8-10 rue Joliot-Curie, 91190, Gif-sur-Yvette, France.
Sci Rep. 2021 Apr 19;11(1):8444. doi: 10.1038/s41598-021-87928-y.
The chemical changes sustained by lignocellulosic biomass during hydrothermal treatment are reflected at multiple scales. This study proposes to benefit from this multiscale nature in order to provide a global understanding of biomass alterations during hydrothermal treatment. For this purpose, complementary imaging techniques-confocal Raman microscopy and X-ray nano-tomography-analysed by image processing and coupled to chemical measurements were used. This unique combination of analyses provided valuable information on topochemical and morphological changes of poplar samples, without the artefacts of sample preparation. At the cell wall level, holocellulose hydrolysis and lignin modifications were observed, which corresponded to anatomical modifications observed at higher scales. Overall, after treatment, samples shrank and had thinner cell walls. When subjected to more severe pre-treatments, cells were disrupted and detached from adjacent cells. Anatomical changes were then used to obtain quantitative indicators of the treatment severity. The effects of treatment at different scales can thus be quantitatively connected in both directions, from micro to macro and from macro to micro.
木质纤维素生物质在水热处理过程中所经历的化学变化在多个尺度上都有体现。本研究旨在利用这种多尺度特性，全面了解生物质在水热处理过程中的变化。为此，本研究采用互补的成像技术——共焦拉曼显微镜和 X 射线纳米断层扫描，并结合图像处理和化学测量进行分析。这种独特的分析组合为杨树木材样本的表面化学和形态变化提供了有价值的信息，而没有样本制备带来的假象。在细胞壁水平上，观察到全纤维素的水解和木质素的修饰，这与在更高尺度上观察到的解剖学变化相对应。总的来说，处理后，样本收缩，细胞壁变薄。当受到更剧烈的预处理时，细胞破裂并从相邻细胞上脱落。然后，利用解剖学变化来获得处理严重程度的定量指标。因此，可以在微观到宏观和宏观到微观的两个方向上定量地连接不同尺度的处理效果。
