Institute of Translational Medicine, Department of Otolaryngology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Health Science Center, Shenzhen 518035, China.
Chem Commun (Camb). 2021 Apr 21;57(31):3753-3756. doi: 10.1039/d1cc00536g. Epub 2021 Mar 22.
We describe an intracellular enzyme-powered DNA circuit probe with a tunable amplifier for sensitive and selective detection of miRNA. This approach has been successfully applied for in situ miRNA-21 fluorescence imaging in live cells. Also, we used chemicals to elevate the APE1 expression level rendering a tunable amplification strength for more flexible imaging applications.
我们描述了一种具有可调放大器的基于细胞内酶的 DNA 电路探针，用于灵敏和选择性检测 miRNA。该方法已成功应用于活细胞中 miRNA-21 的荧光成像。此外，我们还使用化学物质来提高 APE1 的表达水平，从而为更灵活的成像应用提供可调的放大强度。
