基于细胞内酶的 DNA 电路，带有可调放大器，用于 miRNA 成像。
Intracellular enzyme-powered DNA circuit with a tunable amplifier for miRNA imaging.
机构信息
Institute of Translational Medicine, Department of Otolaryngology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Health Science Center, Shenzhen 518035, China.
出版信息
Chem Commun (Camb). 2021 Apr 21;57(31):3753-3756. doi: 10.1039/d1cc00536g. Epub 2021 Mar 22.
We describe an intracellular enzyme-powered DNA circuit probe with a tunable amplifier for sensitive and selective detection of miRNA. This approach has been successfully applied for in situ miRNA-21 fluorescence imaging in live cells. Also, we used chemicals to elevate the APE1 expression level rendering a tunable amplification strength for more flexible imaging applications.
我们描述了一种具有可调放大器的基于细胞内酶的 DNA 电路探针，用于灵敏和选择性检测 miRNA。该方法已成功应用于活细胞中 miRNA-21 的荧光成像。此外，我们还使用化学物质来提高 APE1 的表达水平，从而为更灵活的成像应用提供可调的放大强度。
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