Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Anatomy, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Reprod Sci. 2021 Oct;28(10):2807-2815. doi: 10.1007/s43032-021-00577-4. Epub 2021 Apr 19.
Reactive oxygen species (ROS), involved in the pathogenesis of the polycystic ovary syndrome (PCOS), play a key role in the onset of apoptosis in follicles and granulosa cells (GCs). We aimed to investigate the antioxidant effects of AST and metformin separately and in combination on GCs using a PCOS mouse model. Forty-eight prepubertal female BALB C mice aged 25-30 days and weighing 12-14 g were studied. The PCOS model was created by subcutaneous injection of the dehydroepiandrosterone (DHEA) hormone in 8 mice of BALB C for 20 consecutive days. Apoptosis and the amount of ROS were evaluated in GCs of the ovaries via flow cytometry. The activity of AKT protein was measured by western blot, and the viability of GCs was investigated using spectrophotometry. Ovarian tissue sections were prepared, stained with H&E, and the morphology of the sections was examined. Statistical analysis was performed by SPSS v22.0 software using one-way ANOVA. We found that AST administration leads to a significant reduction in oxidative stress (P<0.01) and consequently a significant decrease in the rate of apoptosis (P<0.01). While the expression of AKT in the AST group revealed a significant increase (P<0.05), it decreased in the metformin group. However, it was still significantly higher than the control and PCOS groups. Ovulation was confirmed in both metformin and AST groups. Further studies are warranted to prove the efficacy of AST and to introduce it as a complementary therapeutic agent in PCOS.
活性氧（ROS）参与多囊卵巢综合征（PCOS）的发病机制，在卵泡和颗粒细胞（GCs）凋亡的发生中起关键作用。我们旨在使用 PCOS 小鼠模型研究 AST 和二甲双胍单独和联合对 GCs 的抗氧化作用。研究了 48 只 25-30 天大、体重 12-14g 的未成熟雌性 BALB C 小鼠。通过在 8 只 BALB C 小鼠的皮下注射脱氢表雄酮（DHEA）激素连续 20 天来创建 PCOS 模型。通过流式细胞术评估卵巢 GCs 中的细胞凋亡和 ROS 量。通过 Western blot 测量 AKT 蛋白的活性，并通过分光光度法研究 GCs 的活力。制备卵巢组织切片，用 H&E 染色，并检查切片的形态。使用 SPSS v22.0 软件通过单因素方差分析进行统计分析。我们发现 AST 给药可显著降低氧化应激（P<0.01），从而显著降低细胞凋亡率（P<0.01）。AST 组的 AKT 表达显著增加（P<0.05），而二甲双胍组则减少。然而，它仍然明显高于对照组和 PCOS 组。在二甲双胍组和 AST 组均确认排卵。需要进一步的研究来证明 AST 的疗效，并将其作为 PCOS 的辅助治疗药物。
