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针对人类副流感病毒 3 型感染的保护性抗体。

Protective antibodies against human parainfluenza virus type 3 infection.

机构信息

Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

Department of Surgery, University of Washington, Seattle, Washington, USA.

出版信息

MAbs. 2021 Jan-Dec;13(1):1912884. doi: 10.1080/19420862.2021.1912884.

DOI:10.1080/19420862.2021.1912884
PMID:33876699
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8078717/
Abstract

Human parainfluenza virus type III (HPIV3) is a common respiratory pathogen that afflicts children and can be fatal in vulnerable populations, including the immunocompromised. There are currently no effective vaccines or therapeutics available, resulting in tens of thousands of hospitalizations per year. In an effort to discover a protective antibody against HPIV3, we screened the B cell repertoires from peripheral blood, tonsils, and spleen from healthy children and adults. These analyses yielded five monoclonal antibodies that potently neutralized HPIV3 . These HPIV3-neutralizing antibodies targeted two non-overlapping epitopes of the HPIV3 F protein, with most targeting the apex. Prophylactic administration of one of these antibodies, PI3-E12, resulted in potent protection against HPIV3 infection in cotton rats. Additionally, PI3-E12 could also be used therapeutically to suppress HPIV3 in immunocompromised animals. These results demonstrate the potential clinical utility of PI3-E12 for the prevention or treatment of HPIV3 in both immunocompetent and immunocompromised individuals.

摘要

人类副流感病毒 3 型（HPIV3）是一种常见的呼吸道病原体，可感染儿童，在免疫功能低下的人群中，包括免疫功能低下的人群，可能是致命的。目前尚无有效的疫苗或治疗方法，每年导致数万人住院。为了发现针对 HPIV3 的保护性抗体，我们从健康儿童和成人的外周血、扁桃体和脾脏中筛选了 B 细胞库。这些分析产生了五种能够有效中和 HPIV3 的单克隆抗体。这些中和 HPIV3 的抗体针对 HPIV3 F 蛋白的两个非重叠表位，大多数针对顶点。预防性给予其中一种抗体 PI3-E12 可有效预防棉鼠感染 HPIV3。此外，PI3-E12 也可用于治疗免疫功能低下的动物中的 HPIV3。这些结果表明 PI3-E12 具有预防或治疗免疫功能正常和免疫功能低下个体中 HPIV3 的临床应用潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c628/8078717/58352762c2b3/KMAB_A_1912884_F0006_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c628/8078717/8acf86930c70/KMAB_A_1912884_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c628/8078717/c6c22debb178/KMAB_A_1912884_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c628/8078717/183e59789d26/KMAB_A_1912884_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c628/8078717/7d68b3caeaba/KMAB_A_1912884_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c628/8078717/f9a465b4e016/KMAB_A_1912884_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c628/8078717/58352762c2b3/KMAB_A_1912884_F0006_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c628/8078717/8acf86930c70/KMAB_A_1912884_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c628/8078717/c6c22debb178/KMAB_A_1912884_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c628/8078717/183e59789d26/KMAB_A_1912884_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c628/8078717/7d68b3caeaba/KMAB_A_1912884_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c628/8078717/f9a465b4e016/KMAB_A_1912884_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c628/8078717/58352762c2b3/KMAB_A_1912884_F0006_OC.jpg

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