Department of Biochemistry and Genome Biology, Hirosaki University Graduate School of Medicine, Aomori, Japan.
CRISPR J. 2021 Apr;4(2):290-300. doi: 10.1089/crispr.2020.0038.
A multitude of molecular interactions with chromatin governs various chromosomal functions in cells. Insights into the molecular compositions at specific genomic regions are pivotal to deepen our understanding of regulatory mechanisms and the pathogenesis of disorders caused by the abnormal regulation of genes. The locus-specific purification of genomic DNA using the clustered regularly interspaced short palindromic repeats (CRISPR) system enables the isolation of target genomic regions for identification of bound interacting molecules. This CRISPR-based DNA purification method has many applications. In this study, we present an overview of the CRISPR-based DNA purification methodologies as well as recent applications.
众多与染色质的分子相互作用控制着细胞中各种染色体功能。深入了解特定基因组区域的分子组成对于加深我们对调控机制的理解以及由基因异常调控引起的疾病的发病机制至关重要。利用成簇规律间隔短回文重复序列 (CRISPR) 系统对基因组 DNA 进行位点特异性纯化，可用于分离靶基因组区域以鉴定结合的相互作用分子。这种基于 CRISPR 的 DNA 纯化方法有许多应用。在本研究中，我们概述了基于 CRISPR 的 DNA 纯化方法以及最近的应用。
