Governmental Institute of Legal Medicine and Forensic Sciences, Turmstraße 21, 10559, Berlin, Germany.
Criminal Investigation Division, Tempelhofer Damm 12, 12101, Berlin, Germany.
Forensic Sci Med Pathol. 2021 Jun;17(2):317-321. doi: 10.1007/s12024-021-00366-0. Epub 2021 Apr 20.
New psychoactive substances are becoming increasingly popular. However, there is a lack of mass spectral information on parent substances and their corresponding metabolites as well as fatal concentrations in body liquids and tissues. Only very few clinical reports and user reports exist. This is also the case for methylaminopropylbenzofuran (MAPB) isomers. Urine from a decedent was screened using different immuno assays, gas chromatography-mass spectrometry (GC-MS), and liquid chromatography-high resolution mass spectrometry (LC-HRMS/MS). Additionally, respective targets were quantitated in all analyzed specimens by LC-MS/MS. Gas chromatography-infrared spectroscopy (GC-IR) was applied to confirm the identity of the intoxication. GC-MS and the amphetamine immuno assay were useful to track the putative intoxication. However, the chemical structure of 2-MAPB was only elucidated by means of LC-HRMS/MS and GC-IR. 2-MAPB was highest in urine (167 µg/mL), followed by gastric content (98.9 µg/mL), bile fluid (30.8 µg/mL), liver (22.2 µg/g), heart blood (16.7 µg/mL), and lowest in femoral blood (7.3 µg/mL). Besides the parent substance, we detected N-demethyl-2-MAPB and hydroxy-2-MAPB in the urine sample. This case report presents an intoxication caused by 2-MAPB. The 2-MAPB concentration found in femoral blood exceeded those reviewed for 5/6-MAPB. The concentrations of the other specimens cannot be evaluated because there exist no comparative data. The values presented can be applied to assess 2-MAPB intoxications in the future.
新精神活性物质越来越受欢迎。然而，关于母体物质及其相应代谢物以及体液和组织中致命浓度的质谱信息却很缺乏。只有极少数的临床报告和用户报告存在。这同样适用于甲基氨基丙基苯并呋喃（MAPB）异构体。使用不同的免疫检测、气相色谱-质谱联用技术（GC-MS）和液相色谱-高分辨率质谱联用技术（LC-HRMS/MS）对死者尿液进行了筛选。此外，还通过 LC-MS/MS 对所有分析样本中的相应靶标进行了定量分析。气相色谱-红外光谱（GC-IR）被用于确认中毒的身份。GC-MS 和安非他命免疫检测可用于追踪潜在的中毒。然而，只有通过 LC-HRMS/MS 和 GC-IR 才能阐明 2-MAPB 的化学结构。2-MAPB 在尿液中的浓度最高（167μg/mL），其次是胃内容物（98.9μg/mL）、胆汁液（30.8μg/mL）、肝（22.2μg/g）、心血（16.7μg/mL），而股血中的浓度最低（7.3μg/mL）。除了母体物质，我们还在尿液样本中检测到 N-去甲基-2-MAPB 和羟基-2-MAPB。本病例报告介绍了一起由 2-MAPB 引起的中毒事件。在股血中检测到的 2-MAPB 浓度超过了 5/6-MAPB 的审查浓度。其他样本的浓度无法评估，因为没有比较数据。所呈现的值可用于评估未来的 2-MAPB 中毒。
