Department of Food Engineering and Technology, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga, Mumbai, 400019, India.
Department of Food Engineering and Technology, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga, Mumbai, 400019, India.
J Environ Manage. 2021 Jul 15;290:112597. doi: 10.1016/j.jenvman.2021.112597. Epub 2021 Apr 17.
This study was aimed to isolate fast-growing thraustochytrids and the influence of seasonal variation in fatty acid composition from the mangrove habitat. The thraustochytrids were isolated from fallen yellowish or green mangrove leaves, in four seasons, including winter, summer, rainy, and post rainy season in one year. The thraustochytrids were analyzed for biomass production, total lipid content, and fatty acid profile. The thraustochytrid isolates showed biomass yield and total lipid content in the range of 14.12 ± 0.69 to 22.98 ± 0.53 g/L and 34.98-58.86% per dry cell weight, respectively. The isolates showed two dominant fatty acids, palmitic acid (PA) as saturated fatty acid (SFA) and docosahexaenoic acid (DHA) as long-chain polyunsaturated fatty acids (LC-PUFA) in total fatty acid (TFA) content. The significant differences (P < 0.05) were observed for seasonal variations in SFA and DHA content in summer isolates and winter isolates. The maximum DHA content with 47.12% of TFA, recorded in winter (January) isolates and summer (April) isolates with SFA 68.82% of TFA. The results from this study were verified the hypothesis that the presence of high DHA producing thraustochytrids in lower temperature season in the same habitat. These findings have also emphasized the role of the environmental temperature conditions and the importance of thraustochytrid fatty acid composition as a dietary biomarker. Also, it revealed the ecological significance of thraustochytrid in DHA enrichment in the food web of the marine ecosystem. These findings could be useful while isolating thraustochytrids according to seasons for industrial application for omega 3 fatty acids and biodiesel production.
本研究旨在从红树林生境中分离出生长迅速的硫丝菌，并研究脂肪酸组成随季节变化的影响。从每年四个季节（冬季、夏季、雨季和雨后季）中落叶的黄化或绿色红树林叶片中分离硫丝菌。分析硫丝菌的生物量生产、总脂质含量和脂肪酸谱。硫丝菌分离株的生物量产量和总脂质含量范围分别为 14.12±0.69 至 22.98±0.53 g/L 和 34.98-58.86%每干细胞重量。分离株总脂肪酸（TFA）中含有两种主要脂肪酸，即棕榈酸（PA）作为饱和脂肪酸（SFA）和二十二碳六烯酸（DHA）作为长链多不饱和脂肪酸（LC-PUFA）。在夏季和冬季分离株中，SFA 和 DHA 含量的季节性变化存在显著差异（P<0.05）。TFA 中 DHA 含量最高为 47.12%，记录于冬季（1 月）和夏季（4 月）分离株中，TFA 中 SFA 含量最高为 68.82%。本研究结果验证了以下假设，即在同一生境中低温季节存在高 DHA 产生的硫丝菌。这些发现还强调了环境温度条件的作用和硫丝菌脂肪酸组成作为饮食生物标志物的重要性。此外，它揭示了硫丝菌在海洋生态系统食物网中 DHA 富集的生态意义。这些发现对于根据季节分离硫丝菌以用于 omega 3 脂肪酸和生物柴油生产的工业应用可能是有用的。
