Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná (UFPR), 81531-970 Curitiba, PR, Brazil.
Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná (UFPR), 81531-970 Curitiba, PR, Brazil.
Bioresour Technol. 2021 Aug;333:125176. doi: 10.1016/j.biortech.2021.125176. Epub 2021 Apr 15.
Omega-3 produced by marine thraustochytrids has appeared as an alternative to fish oil and an eco-friendly solution to overfishing. Herein, an integrative analysis of metagenetics and high-throughput screening was used for bioprospecting marine thraustochytrids from southern Brazil mangrove and coastal seawater. All sampled environments showed biodiversity and abundance of SAR clade. Environmental samples detected with potential lipid-accumulating labyrinthulomycetes were further processed for direct plating and pollen baiting isolation. Microtiter plate system and fluorescence spectroscopy were combined for high-throughput screening of 319 isolates to accumulate lipids. Twenty isolates were selected for submerged cultivation and lipid characterization. Among them, B36 isolate, identified as Aurantiochytrium sp. by 18s rRNA sequencing, achieved the highest biomass (25.60 g/l CDW) and lipids (17.12 g/l CDW). This lipid content had a high biological value with 44.37% LC-PUFAs and 34.6% DHA, which can be used as a sustainable source in vegan, seafood-free and animal feed diets.
海洋甲藻产生的欧米伽-3 已经作为鱼油的替代品出现，是解决过度捕捞的一种环保方法。在此，通过宏基因组学和高通量筛选的综合分析，从巴西南部红树林和沿海水域的海洋甲藻中进行生物勘探。所有采样环境均显示出 SAR 进化枝的生物多样性和丰度。对具有潜在脂质积累旋口虫的环境样本进行直接平板和花粉诱饵分离的进一步处理。微孔板系统和荧光光谱法结合使用，对 319 个分离株进行高通量筛选以积累脂质。选择 20 个分离株进行浸没培养和脂质特性分析。其中，B36 分离株通过 18s rRNA 测序鉴定为 Aurantiochytrium sp.，其生物量（25.60 g/l CDW）和脂质（17.12 g/l CDW）最高。该脂质含量具有较高的生物价值，其中 LC-PUFAs 含量为 44.37%，DHA 含量为 34.6%，可作为纯素食、无海鲜和动物饲料饮食的可持续来源。
