建立一个越南族群诱导多能干细胞系（VRISGi001-A），该细胞系来源于无饲养层系统的脐带血造血干细胞。
Establishment of a Vietnamese ethnicity induced pluripotent stem cell line (VRISGi001-A) from umbilical cord blood hematopoietic stem cells under a feeder-free system.
机构信息
Vinmec Institute of Applied Sciences and Regenerative Medicine (VIASRM), Vinmec Healthcare System, Hanoi, Viet Nam.
Vinmec Institute of Applied Sciences and Regenerative Medicine (VIASRM), Vinmec Healthcare System, Hanoi, Viet Nam; Vinmec Research Institute of Stem Cell and Gene Technology (VRISG), Vinmec Healthcare System, Hanoi, Viet Nam; College of Health Sciences, VinUniversity, Hanoi, Viet Nam.
出版信息
Stem Cell Res. 2021 May;53:102345. doi: 10.1016/j.scr.2021.102345. Epub 2021 Apr 17.
We have established the original footprint-free Vietnamese human induced pluripotent stem cell line, VRISGi001-A, from cord-blood derived CD34 + hematopoietic stem cells (HSCs) of a 35 year old healthy woman under cGMP-compliant process. For the hiPSC induction, three Sendai virus vectors carrying four reprogramming factors including c-MYC, SOX2, KLF4, and OCT3/4 were delivered into CD34 HSCs. The VRISGi001-A cell line expresses the majority of the pluripotent markers and differentiate in vitro into derivatives of three germ layers. The availability of Vietnamese hiPSC line could contribute to the improvement of inadequate genetic diversity in the currently available hiPSC lines.
我们已经建立了原始的无足迹越南人诱导多能干细胞系 VRISGi001-A，该细胞系源自一名 35 岁健康女性脐带血来源的 CD34+造血干细胞（HSCs），符合 cGMP 标准。对于 hiPSC 的诱导，将三个携带四个重编程因子（包括 c-MYC、SOX2、KLF4 和 OCT3/4）的 Sendai 病毒载体递送到 CD34 HSCs 中。VRISGi001-A 细胞系表达大多数多能标志物，并在体外分化为三个胚层的衍生物。越南 hiPSC 系的可用性可能有助于改善目前可用的 hiPSC 系中遗传多样性不足的问题。
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