建立健康个体外周血单核细胞来源的 PLAFMCi004-A 诱导多能干细胞系。
Establishment of PLAFMCi004-A induced pluripotent stem cells derived from PBMCs from a healthy individual.
机构信息
School of Medicine, Nankai University, Tianjin 300071, China; Department of Nephrology, The First Medical Center, Chinese PLA General Hospital, Medical School of Chinese PLA, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing 100853, China.
Clinical Medical School, Guangdong Pharmaceutical University, Guangzhou, China.
出版信息
Stem Cell Res. 2021 May;53:102316. doi: 10.1016/j.scr.2021.102316. Epub 2021 Apr 5.
Peripheral blood mononuclear cells (PBMCs) were isolated from a healthy male individual. PBMCs were reprogrammed into human induced pluripotent stem cells (iPSCs) using plasmids carrying the following reprogramming factors: NANOG, LIN28, OCT4, SOX2, c-MYC, and KLF4. This cell line expressed pluripotency markers, exhibited the normal male karyotype (46, XY), and had the potential to differentiate into 3 germ layers, as confirmed by techniques such as flow cytometry. The iPSC obtained from a healthy individual can be used for organoids to reveal developmental mechanism of tissue and organ regeneration, stem cell therapy and drug screening.
外周血单个核细胞（PBMCs）取自一名健康男性个体。使用携带以下重编程因子的质粒将 PBMCs 重编程为人类诱导多能干细胞（iPSCs）：NANOG、LIN28、OCT4、SOX2、c-MYC 和 KLF4。该细胞系表达多能性标记物，表现出正常的男性核型（46，XY），并且具有分化为 3 个胚层的潜能，如流式细胞术等技术所证实。从健康个体获得的 iPSC 可用于类器官以揭示组织和器官再生、干细胞治疗和药物筛选的发育机制。
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