Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, China.
Department of General Surgery, The 960th Hospital of the PLA Joint Logistics Support Force, Jinan, Shandong 250031, China.
Stem Cell Res. 2021 Oct;56:102540. doi: 10.1016/j.scr.2021.102540. Epub 2021 Sep 16.
Isolated peripheral blood mononuclear cells (PBMCs) from a healthy female were successfully transformed into induced pluripotent stem cells (iPSCs) by non-integrating episomal vectors containing OCT4, SOX2, c-MYC, KLF4, and BCL-XL. The iPSCs displayed a normal karyotype, expressed pluripotency markers and could differentiate into three germ layers in vitro. This iPSC line may be subsequently used for further researches.
从一名健康女性外周血单个核细胞（PBMCs）中成功分离出诱导多能干细胞（iPSCs），这些 iPSCs 是由含有 OCT4、SOX2、c-MYC、KLF4 和 BCL-XL 的非整合性附加体载体转化而来。这些 iPSCs 显示出正常的核型，表达多能性标记物，并能在体外分化为三个胚层。这条 iPSC 系可随后用于进一步的研究。
