建立源自健康个体外周血单个核细胞的诱导多能干细胞系（ZZUSAHi002-A）。
Establishment of an induced pluripotent stem cell line (ZZUSAHi002-A) derived from peripheral blood mononuclear cells of a healthy individual.
作者信息
Liu Shichao, Niu Shaohui, Li Yanfang, Xiong Haiyan, Li Yuan, Jian Liguo, Zhang Lihua
机构信息
Department of Cardiology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Department of Cardiology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
出版信息
Stem Cell Res. 2020 Oct;48:101966. doi: 10.1016/j.scr.2020.101966. Epub 2020 Aug 27.
Peripheral blood mononuclear cells (PBMCs) were isolated from a 32-year-old female and were reprogrammed to human induced pluripotent stem cells (iPSCs) using non-integrative Sendai viral vectors containing reprogramming factors OCT4, SOX2, KLF4 and cMYC. The ZZUSAHi002-A iPSC line expresses pluripotency markers, exhibits a normal female karyotype (46, XX) and has the capacity to differentiate into three germ layers in vivo. This iPSC line may be used for cell transplantations, drug testing and biological tissue engineering.
从一名32岁女性体内分离出外周血单个核细胞（PBMCs），并使用含有重编程因子OCT4、SOX2、KLF4和cMYC的非整合仙台病毒载体将其重编程为人类诱导多能干细胞（iPSCs）。ZZUSAHi002 - A iPSC系表达多能性标志物，具有正常的女性核型（46, XX），并具有在体内分化为三个胚层的能力。该iPSC系可用于细胞移植、药物测试和生物组织工程。
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