Centro de Detección Biomolecular, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico.
Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.
Sci Rep. 2021 Apr 20;11(1):8541. doi: 10.1038/s41598-021-87586-0.
Enteropathogenic E. coli virulence genes are under the control of various regulators, one of which is PerA, an AraC/XylS-like regulator. PerA directly promotes its own expression and that of the bfp operon encoding the genes involved in the biogenesis of the bundle-forming pilus (BFP); it also activates PerC expression, which in turn stimulates locus of enterocyte effacement (LEE) activation through the LEE-encoded regulator Ler. Monomeric PerA directly binds to the per and bfp regulatory regions; however, it is not known whether interactions between PerA and the RNA polymerase (RNAP) are needed to activate gene transcription as has been observed for other AraC-like regulators. Results showed that PerA interacts with the alpha subunit of the RNAP polymerase and that it is necessary for the genetic and phenotypic expression of bfpA. Furthermore, an in silico analysis shows that PerA might be interacting with specific alpha subunit amino acids residues highlighting the direction of future experiments.
肠致病性大肠杆菌的毒力基因受各种调控因子的控制，其中一种是 PerA，一种 AraC/XylS 样调控因子。PerA 直接促进自身表达和编码束形成菌毛 (BFP) 生物发生基因的 bfp 操纵子的表达；它还激活 PerC 的表达，通过 LEE 编码的调控因子 Ler 反过来刺激肠上皮细胞消失 (LEE) 的激活。单体 PerA 直接结合到 per 和 bfp 调控区域；然而，尚不清楚 PerA 是否需要与 RNA 聚合酶 (RNAP) 之间的相互作用来激活基因转录，就像其他 AraC 样调控因子一样。结果表明，PerA 与 RNAP 聚合酶的α亚基相互作用，并且对于 bfpA 的遗传和表型表达是必需的。此外，计算机分析表明 PerA 可能与特定的α亚基氨基酸残基相互作用，突出了未来实验的方向。
