LAQV-REQUIMTE Departamento de Química e Bioquímica, Faculdade de Ciências da Universidade do Porto, Rua do Campo Alegre 687, 4169-007 Porto, Portugal.
Food Funct. 2021 May 21;12(10):4434-4445. doi: 10.1039/d1fo00382h. Epub 2021 Apr 20.
The effect of procyanidin dimer B3, a common food tannin, on the digestion of gliadin proteins was investigated by monitoring the changes in the immunogenic peptides produced during in vitro digestion and immunoreactivity. Interaction studies between procyanidin dimer B3, gluten proteins and/or digestive enzymes were performed by SDS-PAGE. The effect of procyanidin B3 on the enzymatic activity of trypsin, chymotrypsin and pancreatin was evaluated. The differences in the number and nature of immunogenic peptides released during digestion were identified by mass spectrometry. Briefly, the enzymatic activity of gastrointestinal enzymes was only slightly affected but a significant decrease in the immunological properties of the peptides produced during digestion was observed. Overall, although further studies are needed, the interaction between polyphenols and gluten proteins clearly influences gluten protein digestion and immunogenicity, thus suggesting that the consumption of dietary polyphenols can significantly affect the degree of celiac disease downstream immune reactions.
研究了常见的食物单宁原花青素二聚体 B3 对醇溶蛋白消化的影响，通过监测体外消化过程中产生的免疫原性肽的变化和免疫反应来进行研究。通过 SDS-PAGE 研究了原花青素二聚体 B3、面筋蛋白和/或消化酶之间的相互作用。评估了原花青素 B3 对胰蛋白酶、糜蛋白酶和胰酶活性的影响。通过质谱鉴定了消化过程中释放的免疫原性肽的数量和性质的差异。简而言之，胃肠道酶的酶活性仅受到轻微影响，但消化过程中产生的肽的免疫特性显著降低。总的来说，尽管还需要进一步的研究，但多酚和谷蛋白之间的相互作用显然会影响谷蛋白的消化和免疫原性，因此表明食用膳食多酚可以显著影响乳糜泻下游免疫反应的程度。
