Solid-phase radioimmunoassay for detection of antibodies to myelin basic protein.
作者信息
Randolph D H, Kibler R F, Fritz R B
出版信息
J Immunol Methods. 1977;18(3-4):215-24. doi: 10.1016/0022-1759(77)90175-2.
PMID:338832
Abstract
A solid-phase double-antibody radioimmunoassay was developed for the detection of anti-myelin basic protein (BP) in sera. Antigen was adsorbed to glass test tubes, reacted with rat anti-BP sera, followed by 125I-labeled rabbit anti-rat IgG. This assay was capable of detection of specific antibody at low nanogram per ml levels, was technically simple, and the results correlated well with established procedures.
摘要
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