用于检测胶原蛋白抗体的固相放射免疫测定法。
Solid-phase radioimmunoassay for the detection of antibodies to collagen.
作者信息
Clague R B, Brown R A, Weiss J B, Holt P J
出版信息
J Immunol Methods. 1979 May 10;27(1):31-41. doi: 10.1016/0022-1759(79)90236-9.
PMID:458164
Abstract
A solid-phase radioimmunoassay has been developed using polystyrene tubes for the detection of class-specific antibodies to collagen. The optimal conditions for the adsorption of collagen onto the tubes, followed by incubation with the antisera and finally the radiolabelled antibody to the class-specific antibody are described. Studies carried out on antisera raised in rats and rabbits using this assay confirm its sensitivity and applicability.
摘要
已开发出一种使用聚苯乙烯管检测胶原类特异性抗体的固相放射免疫测定法。描述了胶原吸附到管上的最佳条件，随后与抗血清孵育，最后与针对类特异性抗体的放射性标记抗体孵育。使用该测定法对在大鼠和兔子中产生的抗血清进行的研究证实了其敏感性和适用性。
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