凝血酶增强调理酵母聚糖诱导的中性粒细胞化学发光。

Thrombin enhances opsonized zymosan-induced chemiluminescence of neutrophils.

作者信息

Chan K L, Bizios R, Malik A B

机构信息

Department of Physiology, Albany Medical College of Union University, New York 12208.

出版信息

Tissue Cell. 1988;20(1):13-7. doi: 10.1016/0040-8166(88)90003-1.

DOI:10.1016/0040-8166(88)90003-1

PMID:3388412

Abstract

We examined the effects of alpha-thrombin (the native enzyme) on neutrophil activation as assessed by the measurement of chemiluminescence. alpha-Thrombin in physiological concentrations (10(-9)-10(-8)M) did not induce neutrophil chemiluminescence. However, when neutrophils were coincubated with opsonized zymosan and alpha-thrombin, the chemiluminescence response to opsonized zymosan was enhanced in a concentration-dependent manner. The neutrophil chemiluminescence responses to opsonized zymosan and to opsonized zymosan plus alpha-thrombin were dependent on the generation of oxygen-derived free radicals since the chemiluminescence was inhibited by superoxide dismutase. The results indicate that thrombin per se does not induce neutrophil chemiluminescence. However, thrombin enhances the chemiluminescence response to opsonized zymosan suggesting an interaction between thrombin and complement receptors in inducing neutrophil activation. The chemiluminescence response to thrombin and opsonized zymosan is the result of oxygen-derived free radicals.

摘要

我们通过测量化学发光来研究α-凝血酶（天然酶）对中性粒细胞活化的影响。生理浓度（10⁻⁹ - 10⁻⁸M）的α-凝血酶不会诱导中性粒细胞化学发光。然而，当中性粒细胞与调理酵母聚糖和α-凝血酶共同孵育时，对调理酵母聚糖的化学发光反应以浓度依赖的方式增强。中性粒细胞对调理酵母聚糖以及对调理酵母聚糖加α-凝血酶的化学发光反应依赖于氧衍生自由基的产生，因为超氧化物歧化酶可抑制化学发光。结果表明，凝血酶本身不会诱导中性粒细胞化学发光。然而，凝血酶增强了对调理酵母聚糖的化学发光反应，提示凝血酶与补体受体在诱导中性粒细胞活化过程中存在相互作用。对凝血酶和调理酵母聚糖的化学发光反应是氧衍生自由基的结果。