Webster G F, Alexander J C, McArthur W P, Leyden J J
Br J Dermatol. 1984 Jun;110(6):657-63. doi: 10.1111/j.1365-2133.1984.tb04701.x.
Dapsone at doses of 0.5 to 5.0 micrograms/ml was found to produce a dose-dependent inhibition of opsonized zymosan-induced human polymorphonuclear leukocyte (PMN) chemiluminescence (CL) in vitro. Simultaneous exposure of PMN to dapsone and zymosan was as effective in reducing CL as preincubation of PMN with dapsone. Preincubation of PMN with dapsone followed by washing, resulted in the loss of dapsone-mediated CL inhibition, indicating that dapsone did not permanently alter the CL-generating mechanism and that the drug had to be present to inhibit CL. Dapsone did not absorb light at the wavelength of CL and was not toxic to PMN at concentrations tested. Sodium azide, an inhibitor of myeloperoxidase-mediated CL inhibited PMN CL to the same degree as dapsone. When incubated together with PMN, dapsone and azide did not produce an additive inhibition of CL. These data suggest that inhibition of myeloperoxidase may be the mechanism by which dapsone inhibits PMN CL.
研究发现，剂量为0.5至5.0微克/毫升的氨苯砜在体外可对调理酵母聚糖诱导的人多形核白细胞（PMN）化学发光（CL）产生剂量依赖性抑制。PMN同时暴露于氨苯砜和酵母聚糖与PMN预先用氨苯砜孵育在降低CL方面效果相同。PMN预先用氨苯砜孵育后洗涤，导致氨苯砜介导的CL抑制作用丧失，这表明氨苯砜不会永久性改变CL产生机制，且该药物必须存在才能抑制CL。氨苯砜在CL波长处不吸收光，在所测试的浓度下对PMN无毒。叠氮化钠是髓过氧化物酶介导的CL的抑制剂，其对PMN CL的抑制程度与氨苯砜相同。当与PMN一起孵育时，氨苯砜和叠氮化钠不会对CL产生累加抑制作用。这些数据表明，抑制髓过氧化物酶可能是氨苯砜抑制PMN CL的机制。
