Department of General Surgery, University of Health Sciences, Gülhane Training and Research Hospital, Ankara-Turkey.
Ulus Travma Acil Cerrahi Derg. 2021 May;27(3):284-289. doi: 10.14744/tjtes.2020.23460.
In this experimental rat model, we aimed to investigate boric acid's possible protective effect against the formation of post-operative abdominal adhesions through its anti-inflammatory and antioxidant properties.
Forty healthy male albino rats were randomly and evenly allocated to vehicle, hyaluronic acid-based (HA-b) material, boric acid 50 (BA50), boric acid 100 (BA100), and sham groups. Intra-abdominal adhesions were induced by mechanical cecal abrasion. Macroscopic and pathologic assessments of the adhesions were done and tissue tumor necrosis factor-α (TNF-α) and transforming growth factor-β1 (TGF-β1) levels were measured.
Total abdominal adhesion scores were 129.7, 91.07, 53.77, 90.07, and 140.5 for the vehicle, HA-b, BA50, BA100, and sham groups, respectively, with the highest score indicating more severe adhesions. A significant difference in fibrosis scores was noted between both BA50 and BA100, and the sham group (p=0.018). When objective parameters were analyzed, TNF-α levels were significantly lower in the BA50 group than the sham, BA100, and vehicle groups (p=0.01, 0.019, and 0.03, respectively). TGF-β1 levels were also significantly lower in BA50 group than the sham, BA100, and the vehicle groups (p=0.013, 0.016, and 0.05, respectively). No difference was observed for any parameter between BA50 group and HA-b group.
Topical boric acid at a dose of 50 mg/kg is found safe and as effective as the hyaluronic acid-based agent in preventing postoperative abdominal adhesions in our rat model.
在这项实验大鼠模型中，我们旨在通过其抗炎和抗氧化特性，研究硼酸对预防术后腹部粘连形成的可能保护作用。
将 40 只健康雄性白化大鼠随机均分为载体组、透明质酸基（HA-b）材料组、硼酸 50 组（BA50 组）、硼酸 100 组（BA100 组）和假手术组。通过机械性盲肠摩擦诱导腹腔内粘连。对粘连进行宏观和病理评估，并测量组织肿瘤坏死因子-α（TNF-α）和转化生长因子-β1（TGF-β1）水平。
载体组、HA-b 组、BA50 组、BA100 组和假手术组的总腹部粘连评分分别为 129.7、91.07、53.77、90.07 和 140.5，评分越高表示粘连越严重。BA50 组和 BA100 组与假手术组之间的纤维化评分有显著差异（p=0.018）。当分析客观参数时，BA50 组的 TNF-α 水平明显低于假手术组、BA100 组和载体组（p=0.01、0.019 和 0.03）。BA50 组的 TGF-β1 水平也明显低于假手术组、BA100 组和载体组（p=0.013、0.016 和 0.05）。BA50 组与 HA-b 组之间任何参数均无差异。
在我们的大鼠模型中，50mg/kg 的硼酸局部应用是安全的，与透明质酸基制剂一样有效，可预防术后腹部粘连。
