Deployable Technical Analysis Laboratory, Department of National Defence, Ottawa, ON, Canada.
Australia Defence Force, Canberra, ACT, Australia.
J Forensic Sci. 2021 Sep;66(5):1879-1888. doi: 10.1111/1556-4029.14728. Epub 2021 Apr 22.
A trial of rapid DNA (rDNA), a fully automated DNA profiling system, within a technical exploitation (TE) workflow is an important endeavor. In the 2019 Ardent Defender (AD) exercise, the Deployable Technical Analysis Laboratory (DTAL), of the Canadian Department of National Defence (DND), evaluated the use of rDNA using ANDE™. Sixteen samples were processed during a pre-exercise "controlled" setting, 44 samples were from an "uncontrolled" environment during the exercise, and 22 samples were buccal swabs. The proportion of profiles suitable for upload to ANDE™ was 95.5% of buccal samples (21/22), 66.7% controlled samples, and 15.9% for uncontrolled samples. A considerable difference was observed in the proportions of complete DNA profiles obtained from all exploited items between the controlled (58.3%) and uncontrolled (15.9%) trials and in the proportions of samples where no DNA was detected (16.7% controlled trial vs. 56.8% uncontrolled trial). Overall, the trials highlighted the potential to gain identity intelligence using rDNA within a TE workflow and revealed the impact of operational constraints and the need to improve certain TE practices to gain the most benefit from rDNA. It also demonstrated the benefit of including an uncontrolled component for a more realistic indication of rDNA effectiveness in operational settings and highlighted operational practices impacting rDNA success. Mixture deconvolution was difficult as current guidelines do not consider some of the stochastic effects produced by the rDNA analysis; however, overall, the study demonstrated that rDNA using the ANDE™ instrument could be successfully incorporated into a TE workflow within a deployable laboratory.
在技术开发（TE）工作流程中尝试快速 DNA（rDNA），这是一种完全自动化的 DNA 分析系统，是一项重要的工作。在 2019 年的“坚定捍卫者”（AD）演习中，加拿大国防部（DND）的可部署技术分析实验室（DTAL）使用 ANDE™评估了 rDNA 的使用情况。在演习前的“受控”环境中处理了 16 个样本，在演习期间的“不受控”环境中处理了 44 个样本，还有 22 个样本是口腔拭子。适合上传到 ANDE™的样本比例为 95.5%（21/22）的口腔样本、66.7%的受控样本和 15.9%的不受控样本。在受控（58.3%）和不受控（15.9%）试验中，从所有开发利用的物品中获得完整 DNA 谱的比例以及未检测到 DNA 的样本比例（16.7%的受控试验与 56.8%的不受控试验）之间存在相当大的差异。总体而言，这些试验突显了在 TE 工作流程中使用 rDNA 获得身份情报的潜力，并揭示了操作限制的影响以及需要改进某些 TE 实践以从 rDNA 中获得最大收益的必要性。它还证明了在更现实的操作环境中包含不受控组件对于 rDNA 有效性的指示的益处，并突出了影响 rDNA 成功的操作实践。混合物解析困难，因为当前的指南没有考虑到 rDNA 分析产生的一些随机效应；然而，总的来说，该研究表明，使用 ANDE™仪器的 rDNA 可以成功地纳入可部署实验室的 TE 工作流程中。
