Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University/UNESP, São José dos Campos, SP, Brazil.
Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University/UNESP, São José dos Campos, SP, Brazil.
Photodiagnosis Photodyn Ther. 2021 Jun;34:102303. doi: 10.1016/j.pdpdt.2021.102303. Epub 2021 Apr 19.
Photodynamic therapy (PDT) is a promising strategy to control cariogenic pathogens, such as Streptococcus mutans. Seeking to reach the total bacterial elimination from dental surfaces, novel photosensitizers have been investigated, such as Fotoenticine (FTC) derived from chlorin e6. The objective of this study was to investigate the photodynamic effects of FTC against several clinical strains of S. mutans. Clinical isolates were obtained from patients with active carious lesions, identified by molecular analysis and subjected to PDT using laser irradiation (660 nm and 39.5 J/cm) in planktonic and biofilm stages. We identified 11 S. mutans strains from cervical, occlusal and proximal caries. PDT mediated by FTC has totally eliminated the S. mutans cells in planktonic growth for all analyzed strains. In biofilms, PDT with FTC reached statistically significant reductions compared with the non-treated control group, at 5.4, 5.5 and 6.5 Log (CFU/mL), respectively, for the strains from proximal, occlusal and cervical caries. The scanning electron microscopy evaluations confirmed that PDT mediated by FTC was able to disaggregate and kill the S. mutans cells adhered to enamel surface, suggesting its potential to disinfect the dental tissues.
光动力疗法（PDT）是一种很有前途的控制致龋病原体的策略，如变形链球菌。为了达到从牙面彻底清除细菌的目的，研究人员探索了一些新的光敏剂，如来源于氯己定的 Fotoenticine（FTC）。本研究旨在探讨 FTC 对几种临床分离的变形链球菌的光动力作用。从患有活动性龋损的患者中分离出临床分离株，通过分子分析进行鉴定，并在浮游和生物膜阶段用激光照射（660nm 和 39.5J/cm）进行 PDT。我们从颈龋、窝沟龋和邻面龋中鉴定出 11 株变形链球菌。对于所有分析的菌株，FTC 介导的 PDT 完全消除了浮游生长中的变形链球菌细胞。在生物膜中，与未经处理的对照组相比，FTC 的 PDT 在 5.4、5.5 和 6.5Log（CFU/mL）分别达到了统计学显著降低，分别来自近中面龋、窝沟龋和颈面龋的菌株。扫描电子显微镜评估证实，FTC 介导的 PDT 能够使黏附在釉质表面的变形链球菌细胞解聚和死亡，表明其有消毒牙体组织的潜力。
