Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University/UNESP, São José dos Campos, SP, Brazil.
Fotobiologia Aplicada à Saúde (FOTOBIOS), Instituto de Pesquisa e Desenvolvimento (IP&D), Universidade do Vale do Paraíba/UNIVAP, São José dos Campos, SP, Brazil.
Photodiagnosis Photodyn Ther. 2020 Dec;32:102001. doi: 10.1016/j.pdpdt.2020.102001. Epub 2020 Sep 19.
Chitosan (CS), a biopolymer with intrinsic antimicrobial activity, can increase antimicrobial photodynamic inactivation (aPDI). The aim of this study was to evaluate the capacity of CS to potentiate the efficacy of Photoditazine® (PDZ)-mediated aPDI of the cariogenic bacterium Streptococcus mutans. CS effectively augmented the effects of aPDI, with reductions of approximately 4.5 logs in both planktonic and biofilm states. The combined treatment was also capable of reducing the number of S. mutans cells and amount of extracellular matrix in biofilms formed on enamel surfaces, which were characterized using scanning electron microscopy analysis. Furthermore, CS increased the absorption of PDZ by S. mutans cells. The combination of CS with PDZ-mediated aPDI is hence a promising antimicrobial approach against S. mutans and may be useful to control dental caries.
壳聚糖（CS）是一种具有内在抗菌活性的生物聚合物，可增强抗菌光动力失活（aPDI）。本研究旨在评估 CS 增强致龋菌变形链球菌 Photoditazine®（PDZ）介导的 aPDI 效果的能力。CS 可有效增强 aPDI 的效果，在浮游和生物膜状态下减少约 4.5 个对数级。联合治疗还能够减少釉质表面生物膜中变形链球菌细胞的数量和细胞外基质的量，这通过扫描电子显微镜分析进行了表征。此外，CS 增加了 PDZ 被变形链球菌细胞的吸收。因此，CS 与 PDZ 介导的 aPDI 的联合使用是一种有前途的抗变形链球菌的抗菌方法，可能有助于控制龋齿。
