一种体外测定牛多形核白细胞吞噬活性的简单方法。

A simple method for the in vitro determination of phagocytic activity of bovine polymorphonuclear leukocytes.

作者信息

Soler-Rodríguez A M, Armas J M

机构信息

Universidad Simón Bolívar, Dept. Biología Celular, Caracas, Venezuela.

出版信息

Vet Immunol Immunopathol. 1988 Mar;18(2):139-47. doi: 10.1016/0165-2427(88)90056-6.

DOI:10.1016/0165-2427(88)90056-6

PMID:3388761

Abstract

A simple method for the in vitro determination of the phagocytic activity of bovine polymorphonuclear leukocytes (PMNL) is described. An enriched PMNL population was obtained from peripheral blood of healthy cattle and mixed with opsonized Candida utilis or with opsonized bovine red blood cells on BSA treated coverglass slides. The phagocytosis and killing of C. utilis was determined simultaneously on the coverglass slides stained with acridine orange. Phagocytosis of bovine erythrocytes was determined by microscopic examination of the Wright-Giemsa stained slides. This method is appropriate under limited laboratory conditions because it does not require highly sophisticated equipment and materials, it is easy and rapid to perform, reproducible and inexpensive. Therefore, it could be used as a first evaluation of the phagocytic activity of bovine PMNL.

摘要

本文描述了一种体外测定牛多形核白细胞（PMNL）吞噬活性的简单方法。从健康牛的外周血中获得富集的PMNL群体，并将其与经调理的产朊假丝酵母或经调理的牛红细胞在经牛血清白蛋白处理的盖玻片上混合。在经吖啶橙染色的盖玻片上同时测定产朊假丝酵母的吞噬作用和杀伤作用。通过对经瑞氏-吉姆萨染色的玻片进行显微镜检查来测定牛红细胞的吞噬作用。该方法在有限的实验室条件下适用，因为它不需要高度复杂的设备和材料，操作简便、快速，具有可重复性且成本低廉。因此，它可用于对牛PMNL吞噬活性的初步评估。