培养牛多形核白细胞（PMNL）的单层系统：长期培养对细胞活力和吞噬功能的影响。
Monolayer system of cultivating bovine polymorphonuclear leukocytes (PMNL): effect of long-term cultivation on the viability and phagocytic function of the cells.
作者信息
Gyang E O, Maheshwaran S K, Usenik E A
出版信息
Vet Res Commun. 1987;11(3):205-9. doi: 10.1007/BF00570917.
PMID:3629942
Abstract
A monolayer system of cultivating bovine PMNL is described. Poly-L-Lysine (PLL) significantly (P less than 0.01) enhanced the attachment of bovine PMNL to glass. Long-term cultivation up to seven days, in a mixture of calf serum and RPMI 1640, had no effect on the viability of the cells, but cultivation for 3 days or more significantly reduced the ability of the PMNL to phagocytose bacteria.
摘要
描述了一种培养牛多形核白细胞（PMNL）的单层系统。聚-L-赖氨酸（PLL）显著（P<0.01）增强了牛PMNL与玻璃的附着。在小牛血清和RPMI 1640的混合物中进行长达七天的长期培养对细胞活力没有影响，但培养三天或更长时间会显著降低PMNL吞噬细菌的能力。
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