Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Poultry lnstitute, Huadu Yukou Poultry Industry Co. Ltd., Beijing 101206, China.
Poult Sci. 2021 Jun;100(6):101087. doi: 10.1016/j.psj.2021.101087. Epub 2021 Mar 4.
In birds, the sperm storage tubules (SST) are dispersed in uterovaginal junction (UVJ) and highly correlated with differential capacity of sperm storage (SS) in and among species with unspecified mechanisms. Here, the SS duration of 252 egg layer breeders was evaluated in 5 rounds with 3 phenotypic traits to screen high- and low-SS individuals, respectively, followed with transcriptome of UVJ tissues and metabolome of serum (high-SS vs. low-SS) to decipher the candidate genes and biochemical markers correlated with differential SS capacity. Histological characterization suggested slightly higher density of SST in UVJ (high-SS vs. low-SS). Transcriptome analyses identified 596 differentially expressed genes (336 upregulated vs. 260 downregulated), which were mainly enriched in gene ontology terms of homeostasis, steroid and lipid metabolism and hormone activity, and 12 significant pathways (P < 0.05) represented by calcium, steroid, and lipid metabolism. Immunohistochemical staining of GNAQ, ST6GAL1, ADFP, and PCNA showed similar distribution in UVJ tissues between 2 groups. Several candidates (HSD11B2, DIO2, AQP3, GNAQ, NANS, ST6GAL1) combined with 4 (11β-prostaglandin F2α, prostaglandin B1, 7α-hydroxytestosterone, and N-acetylneuraminic acid) of 40 differential metabolites enriched in serum metabolome were considered as regulators and biomarkers of SS duration in egg layer breeders. The integrated transcriptome and metabolome analyses of chicken breeder hens will provide novel insights for exploration and improvement of differential SS capacity in birds.
在鸟类中，精子储存小管（SST）分散在阴道子宫交界处（UVJ）中，与物种间和物种内不同的精子储存能力（SS）高度相关，但具体机制尚不清楚。本研究通过 5 轮实验评估了 252 只蛋鸡的 SS 持续时间，分别使用 3 个表型特征筛选高 SS 和低 SS 个体，然后对 UVJ 组织的转录组和血清代谢组（高 SS 与低 SS）进行分析，以揭示与 SS 能力差异相关的候选基因和生化标志物。组织学特征表明，UVJ 中的 SST 密度略高（高 SS 与低 SS）。转录组分析鉴定了 596 个差异表达基因（336 个上调，260 个下调），这些基因主要富集在同源性、类固醇和脂质代谢以及激素活性的基因本体术语中，12 个显著通路（P<0.05）由钙、类固醇和脂质代谢代表。GNAQ、ST6GAL1、ADFP 和 PCNA 的免疫组织化学染色显示，2 组 UVJ 组织中的分布相似。几个候选基因（HSD11B2、DIO2、AQP3、GNAQ、NANS、ST6GAL1）与血清代谢组中富集的 40 个差异代谢物中的 4 个（11β-前列腺素 F2α、前列腺素 B1、7α-羟睾酮和 N-乙酰神经氨酸）结合，被认为是蛋鸡 SS 持续时间的调控因子和生物标志物。鸡种母鸡的综合转录组和代谢组分析将为探索和改善鸟类的 SS 能力提供新的思路。
