Nasseri Ehsan Baradaran, Eskandarizadeh Ali
Department of Restorative and Cosmetic Dentistry, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran.
Oral and Dental Diseases Research Center, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran.
Dent Res J (Isfahan). 2020 Dec 10;17(6):424-432. eCollection 2020 Nov-Dec.
Dentin-bonding agents should have good biocompatibility as they come into close and prolonged contact with vital dentin. The present study aimed to evaluate and compare the cytotoxicity of total etch and self-etch dentin bonding systems with two different dentin thickness on L929 cell line.
In this study 80 Class I cavities were prepared on the occlusal surfaces. The teeth were randomly divided to two groups of 40 each based on two RDT 0.5 mm and 1.5 mm. Samples were further subdivided into four subgroups of 10. Group 1: Adper Scotch bond Multi-Purpose (SBMP), Group 2: Adper Single Bond Plus (SBP), Group 3: Adper Scotch bond SE (SSE) and Group 4: Adper Easy One (EO). Group 1 and 2 were total-etch and Group 3 and 4 were self-etch. The cavities were sealed after applying of dentin bonding. Then crowns were immersed in culture medium for 24 hours and the cytotoxicity of resultant toxic extraction was measured with 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in 4 serial dilutions (neat,1/2 ,1/10 ,1/100). Data were analyzed by Two-way ANOVA and -test.
For all the dilutions, cytotoxicity was significantly higher with 0.5 mm remaining dentin thickness (RDT) relative to 1.5 mm RDT ( ≤ 0.05). In neat dilution for both RDTs, cytotoxicity was different among all the four dentin bondings. Expression in decreasing order of cytotoxicity was SBP > SBMP > SSE > EO for both RDTs in neat dilution ( < 0.05). For all the dilutions, cytotoxicity was significantly lower for self-etch bonding systems in comparison with total-etch bonding systems ( < 0.05).
In the present study, lower cytotoxicity was found with an increase in the dilution of toxic extract and also cytotoxicity decreased with an increased dentin thickness. The adhesive systems had degrees of cytotoxic effects on cultured L929 compared to the control, except for the EO group.
牙本质粘结剂与活髓牙本质密切且长期接触，应具有良好的生物相容性。本研究旨在评估和比较全酸蚀和自酸蚀牙本质粘结系统在两种不同牙本质厚度下对L929细胞系的细胞毒性。
本研究在80颗牙齿的咬合面制备I类洞。根据剩余牙本质厚度（RDT）0.5mm和1.5mm将牙齿随机分为两组，每组40颗。样本进一步细分为四个亚组，每组10颗。第1组：Adper Scotch bond多功能粘结剂（SBMP），第2组：Adper Single Bond Plus粘结剂（SBP），第3组：Adper Scotch bond自酸蚀粘结剂（SSE），第4组：Adper Easy One粘结剂（EO）。第1组和第2组为全酸蚀粘结剂，第3组和第4组为自酸蚀粘结剂。应用牙本质粘结剂后封闭窝洞。然后将牙冠浸入培养基中24小时，并用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐（MTT）法测定所得毒性提取物在4个系列稀释度（原液、1/2、1/10、1/100）下的细胞毒性。数据采用双向方差分析和t检验进行分析。
对于所有稀释度，剩余牙本质厚度（RDT）为0.5mm时的细胞毒性显著高于1.5mm RDT时的细胞毒性（P≤0.05）。在两种RDT的原液稀释度下，所有四种牙本质粘结剂的细胞毒性均不同。在原液稀释度下，两种RDT的细胞毒性从高到低依次为SBP＞SBMP＞SSE＞EO（P＜0.05）。对于所有稀释度，自酸蚀粘结系统的细胞毒性显著低于全酸蚀粘结系统（P＜0.05）。
在本研究中，发现毒性提取物稀释度增加时细胞毒性降低，且随着牙本质厚度增加细胞毒性也降低。除EO组外，与对照组相比，粘结系统对培养的L929细胞均有一定程度的细胞毒性作用。
