National Research Council - Research Institute on Terrestrial Ecosystems, Section of Pisa, Via Moruzzi, 1, 56124, Pisa, Italy.
Eni S.p.A., Renewable Energy & Environmental Laboratories, S. Donato Milanese, MI, Italy.
Environ Sci Pollut Res Int. 2021 Sep;28(34):47294-47305. doi: 10.1007/s11356-021-14074-3. Epub 2021 Apr 23.
Phytoextraction is currently investigated to effectively remediate soil contaminated by metals and provide highly competitive biomass for energy production. This research aimed to increase arsenic (As) removal from contaminated soil using industrial Cannabis sativa L., a suitable energy crop for biofuel production. Assisted phytoextraction experiments were conducted on a microcosm scale to explore the ability of two friendly treatments, sodium sulphate (SO) and exogenous cytokinin (CK), in increasing As phytoextraction efficiency. The results showed that the treatments significantly increased As phytoextraction. Cytokinin was the most effective agent for effectively increasing translocation and the amount of As in aerial parts of C. sativa. In fact, the concentration of As in the shoots of CK-treated plants increased by 172% and 44% compared to untreated and SO-treated plants, respectively. However, the increased As amount accumulated in C. sativa tissues due to the two treatments negatively affected plant growth. Arsenic toxicity caused a significant decrease in aerial C. sativa biomass treated with CK and SO of about 32.7% and 29.8% compared to untreated plants, respectively. However, for our research purposes, biomass reduction has been counterbalanced by an increase in As phytoextraction, such as to consider C. sativa and CK an effective combination for the remediation of As-contaminated soils. Considering that C. sativa has the suitable characteristics to provide valuable resources for bioenergy production, our work can help improve the implementation of a sustainable management model for As contaminated areas, such as phytoremediation coupled with bioenergy generation.
植物提取目前被研究用于有效修复受金属污染的土壤，并提供用于能源生产的高竞争力生物质。本研究旨在使用工业大麻（Cannabis sativa L.）增加受污染土壤中砷（As）的去除，大麻是生物燃料生产的合适能源作物。在微宇宙规模上进行了辅助植物提取实验，以探索两种友好处理方法，硫酸钠（SO）和外源细胞分裂素（CK），在提高 As 植物提取效率方面的能力。结果表明，这些处理方法显著提高了 As 的植物提取效率。细胞分裂素是最有效的有效增加向性和大麻地上部分 As 含量的药剂。事实上，与未处理和 SO 处理的植物相比，CK 处理植物的地上部分 As 浓度分别增加了 172%和 44%。然而，由于两种处理方法，As 含量的增加会对植物生长产生负面影响。砷毒性导致 CK 和 SO 处理的大麻地上部分生物量分别比未处理的植物减少了约 32.7%和 29.8%。然而，就我们的研究目的而言，由于 CK 和 SO 增加了 As 的植物提取，生物量的减少得到了补偿，这使得大麻和 CK 成为受 As 污染土壤修复的有效组合。考虑到大麻具有提供生物能源生产有价值资源的合适特性，我们的工作可以帮助改善受 As 污染地区的可持续管理模型的实施，例如与生物能源生产相结合的植物修复。
