Department of Orthopaedic Surgery, Liaocheng People's Hospital, Liaocheng, China.
Department of Anesthesiology, Liaocheng People's Hospital Dongchangfu District, Liaocheng, China.
J Biol Regul Homeost Agents. 2021 Mar-Apr;35(2):593-603. doi: 10.23812/21-40-A.
Autologous chondrocyte (CH) transplantation is a novel strategy to treat post-traumatic osteoarthritis (PTOA). In this study, an in vitro coculture model was used to explore the effects of interleukin (IL)-10 overexpressed CHs on degenerated CHs. The original CHs were isolated from the patients' knee joint cartilage and pretreated with IL-1β to get degenerated CHs. Moreoer, CHs were transfected with a lentivirus vector to overexpress IL-10. After coculture with the degenerated CHs, the apoptosis, collagen X, IL-6, and TNF-α of original CHs were increased, and the collagen II and IL-10 were decreased compared to the separated culture condition. Coculture with original CHs did not alleviate the degeneration of the IL-1β-pretreated CHs. However, coculture with the IL-10-overexpressed CHs rescued the proliferation, collagen II, aggrecan, SOX9, and IL-10 expression, and suppressed the apoptosis, collagen X, RUnx2, IL-6, and TNF-α levels in the IL-1β pretreated CHs. Additionally, the IL-10-overexpressed CHs also maintained a healthy state when cocultured with the degenerated CHs. Therefore, transplanting the IL-10-overexpressed CHs in the treatment of PTOA would obtain a more durable and visible effect in alleviating the CH degeneration.
自体软骨细胞（CH）移植是治疗创伤后骨关节炎（PTOA）的一种新策略。在这项研究中，使用体外共培养模型来探索过表达白细胞介素（IL）-10 的 CH 对退变 CH 的影响。原代 CH 从患者膝关节软骨中分离出来，并经 IL-1β预处理以获得退变 CH。此外，CH 用慢病毒载体转染以过表达 IL-10。与退变 CH 共培养后，与分离培养条件相比，原代 CH 的凋亡、胶原 X、IL-6 和 TNF-α增加，而胶原 II 和 IL-10 减少。与原代 CH 共培养不能减轻 IL-1β预处理 CH 的退变。然而，与过表达 IL-10 的 CH 共培养可挽救 IL-1β 预处理 CH 的增殖、胶原 II、聚集蛋白聚糖、SOX9 和 IL-10 表达，并抑制凋亡、胶原 X、Runx2、IL-6 和 TNF-α水平。此外，过表达 IL-10 的 CH 与退变 CH 共培养时仍保持健康状态。因此，在 PTOA 的治疗中移植过表达 IL-10 的 CH 将获得更持久和明显的缓解 CH 退变的效果。
